Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion

In spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in wh...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Ferrero, Mariana C., Fossati, Carlos A., Rumbo, Martín, Baldi, Pablo C.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Bioquímica
Brucella
Gut epithelial cells
Inflammatory response
Intracellular replication
Enfermedades Inflamatorias del Intestino
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84395
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84395
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Brucella
Gut epithelial cells
Inflammatory response
Intracellular replication
Enfermedades Inflamatorias del Intestino
spellingShingle Bioquímica
Brucella
Gut epithelial cells
Inflammatory response
Intracellular replication
Enfermedades Inflamatorias del Intestino
Ferrero, Mariana C.
Fossati, Carlos A.
Rumbo, Martín
Baldi, Pablo C.
Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
topic_facet Bioquímica
Brucella
Gut epithelial cells
Inflammatory response
Intracellular replication
Enfermedades Inflamatorias del Intestino
description In spite of the frequent acquisition of Brucella infection by the oral route in humans, the interaction of the bacterium with cells of the intestinal mucosa has been poorly studied. Here, we show that different Brucella species can invade human colonic epithelial cell lines (Caco-2 and HT-29), in which only smooth species can replicate efficiently. Infection with smooth strains did not produce a significant cytotoxicity, while the rough strain RB51 was more cytotoxic. Infection of Caco-2 cells or HT-29 cells with either smooth or rough strains of Brucella did not result in an increased secretion of TNF-α, IL-1β, MCP-1, IL-10 or TGF-β as compared with uninfected controls, whereas all the infections induced the secretion of IL-8 and CCL20 by both cell types. The MCP-1 response to flagellin from Salmonella typhimurium was similar in Brucella-infected or uninfected cells, ruling out a bacterial inhibitory mechanism as a reason for the weak proinflammatory response. Infection did not modify ICAM-1 expression levels in Caco-2 cells, but increased them in HT-29 cells. These results suggest that Brucella induces only a weak proinflammatory response in gut epithelial cells, but produces a significant CCL20 secretion. The latter may be important for bacterial dissemination given the known ability of Brucella to survive in dendritic cells.
format Articulo
Articulo
author Ferrero, Mariana C.
Fossati, Carlos A.
Rumbo, Martín
Baldi, Pablo C.
author_facet Ferrero, Mariana C.
Fossati, Carlos A.
Rumbo, Martín
Baldi, Pablo C.
author_sort Ferrero, Mariana C.
title Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title_short Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title_full Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title_fullStr Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title_full_unstemmed Brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant CCL20 secretion
title_sort brucella invasion of human intestinal epithelial cells elicits a weak proinflammatory response but a significant ccl20 secretion
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/84395
work_keys_str_mv AT ferreromarianac brucellainvasionofhumanintestinalepithelialcellselicitsaweakproinflammatoryresponsebutasignificantccl20secretion
AT fossaticarlosa brucellainvasionofhumanintestinalepithelialcellselicitsaweakproinflammatoryresponsebutasignificantccl20secretion
AT rumbomartin brucellainvasionofhumanintestinalepithelialcellselicitsaweakproinflammatoryresponsebutasignificantccl20secretion
AT baldipabloc brucellainvasionofhumanintestinalepithelialcellselicitsaweakproinflammatoryresponsebutasignificantccl20secretion
bdutipo_str Repositorios
_version_ 1764820488678277120

Ejemplares similares