Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance

The isolation of rhizobial strains which exhibit an intrinsic tolerance to acidic conditions has been reported and has facilitated studies on the basic mechanisms underlying acid tolerance. <i>Rhizobium tropici</i> strain CIAT899 displays a high intrinsic tolerance to acidity and therefo...

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Detalles Bibliográficos
Autores principales: Riccillo, Pablo Miguel, Muglia, Cecilia Isabel, Bruijn, Frans J. de, Roe, Andrew J., Booth, Ian R., Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Exactas
Rhizobium tropici
acidity
glutathione
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84378
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84378
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Rhizobium tropici
acidity
glutathione
spellingShingle Ciencias Exactas
Rhizobium tropici
acidity
glutathione
Riccillo, Pablo Miguel
Muglia, Cecilia Isabel
Bruijn, Frans J. de
Roe, Andrew J.
Booth, Ian R.
Aguilar, Orlando Mario
Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance
topic_facet Ciencias Exactas
Rhizobium tropici
acidity
glutathione
description The isolation of rhizobial strains which exhibit an intrinsic tolerance to acidic conditions has been reported and has facilitated studies on the basic mechanisms underlying acid tolerance. <i>Rhizobium tropici</i> strain CIAT899 displays a high intrinsic tolerance to acidity and therefore was used in this work to study the molecular basis of bacterial responses to acid conditions and other environmental stresses. We generated a collection of <i>R. tropici</i> CIAT899 mutants affected in acid tolerance using Tn<i>5-luxAB</i> mutagenesis, and one mutant strain (CIAT899-13T2), which fails to grow under acid conditions, was characterized in detail. Strain CIAT899-13T2 was found to contain a single Tn<i>5-luxAB</i> insertion in a gene showing a high degree of similarity with the <i>Escherichia coli gshB</i> gene, encoding the enzyme glutathione synthetase. Intracellular potassium pools and intracellular pH levels were found to be lower in the mutant than in the parent. The glutathione-deficient mutant was shown to be sensitive to weak organic acids, osmotic and oxidative stresses, and the presence of methylglyoxal. Glutathione restores responses to these stresses almost to wild-type levels. Our data show that in <i>R. tropici</i> the production of glutathione is essential for growth in extreme environmental conditions. The mutant strain CIAT899-13T2 induced effective nodules; however, it was found to be outcompeted by the wild-type strain in coinoculation experiments.
format Articulo
Articulo
author Riccillo, Pablo Miguel
Muglia, Cecilia Isabel
Bruijn, Frans J. de
Roe, Andrew J.
Booth, Ian R.
Aguilar, Orlando Mario
author_facet Riccillo, Pablo Miguel
Muglia, Cecilia Isabel
Bruijn, Frans J. de
Roe, Andrew J.
Booth, Ian R.
Aguilar, Orlando Mario
author_sort Riccillo, Pablo Miguel
title Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance
title_short Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance
title_full Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance
title_fullStr Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance
title_full_unstemmed Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance
title_sort glutathione is involved in environmental stress responses in rhizobium tropici, including acid tolerance
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/84378
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AT boothianr glutathioneisinvolvedinenvironmentalstressresponsesinrhizobiumtropiciincludingacidtolerance
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bdutipo_str Repositorios
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