Islet neogenesis: An apparent key component of long-term pancreas adaptation to increased insulin demand

This study aimed to determine the relative importance of different functional and morphological pancreatic changes induced by the chronic administration of a sucrose-rich diet (SRD) to maintain normal glucose homeostasis. Male Wistar rats were fed either sucrose (SRD) or starch (CD) for 6 and 12 mon...

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Autores principales: Del Zotto, Héctor Herminio, Borelli, María Inés, Flores, Luis Emilio, García, María Elisa, Gómez Dumm, César Leandro Alberto, Chicco, A., Lombardo, Y. B., Gagliardino, Juan José
Formato: Articulo
Lenguaje:Inglés
Publicado: 2004
Materias:
Ciencias Médicas
Insulina
Páncreas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84370
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84370
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Insulina
Páncreas
spellingShingle Ciencias Médicas
Insulina
Páncreas
Del Zotto, Héctor Herminio
Borelli, María Inés
Flores, Luis Emilio
García, María Elisa
Gómez Dumm, César Leandro Alberto
Chicco, A.
Lombardo, Y. B.
Gagliardino, Juan José
Islet neogenesis: An apparent key component of long-term pancreas adaptation to increased insulin demand
topic_facet Ciencias Médicas
Insulina
Páncreas
description This study aimed to determine the relative importance of different functional and morphological pancreatic changes induced by the chronic administration of a sucrose-rich diet (SRD) to maintain normal glucose homeostasis. Male Wistar rats were fed either sucrose (SRD) or starch (CD) for 6 and 12 months. At both periods, serum glucose and triacylglycerol levels were significantly higher (P<0.05; paired and unpaired Student's t-test) in SRD rats. Serum insulin levels were significantly lower in SRD only at 12 months. At 6 months, the insulin secretion dose-response curve in SRD rats showed a shift to the left that was no longer observed at 12 months, when SRD islets decreased their response to 16 mM glucose. At 6 months, SRD rats showed a significant increase in β-cell volume density (Vvi) and islet cell replication rate, together with a decrease in β-cell apoptotic rate. Changes were not detected in the percentage of PDX-1- and islet neogenesis associated protein (INGAP)-positive cells. Conversely, at 12 months, there was a significant decrease in β-cell Vvi and in the percentage of PDX-1-positive cells; the islet cell replication rate was not modified, and the number of apoptotic β-cells increased significantly. No signs of increased neogenesis or INGAP-positive cells were recorded at any period in SRD rats. Our results show that SRD rats are unable to develop functional and morphological pancreatic reactive changes sufficient to maintain normal glucose and triacylglycerol levels for a long period. Such failure could be ascribed to their inability to increase the rate of neogenesis and of INGAP production.
format Articulo
Articulo
author Del Zotto, Héctor Herminio
Borelli, María Inés
Flores, Luis Emilio
García, María Elisa
Gómez Dumm, César Leandro Alberto
Chicco, A.
Lombardo, Y. B.
Gagliardino, Juan José
author_facet Del Zotto, Héctor Herminio
Borelli, María Inés
Flores, Luis Emilio
García, María Elisa
Gómez Dumm, César Leandro Alberto
Chicco, A.
Lombardo, Y. B.
Gagliardino, Juan José
author_sort Del Zotto, Héctor Herminio
title Islet neogenesis: An apparent key component of long-term pancreas adaptation to increased insulin demand
title_short Islet neogenesis: An apparent key component of long-term pancreas adaptation to increased insulin demand
title_full Islet neogenesis: An apparent key component of long-term pancreas adaptation to increased insulin demand
title_fullStr Islet neogenesis: An apparent key component of long-term pancreas adaptation to increased insulin demand
title_full_unstemmed Islet neogenesis: An apparent key component of long-term pancreas adaptation to increased insulin demand
title_sort islet neogenesis: an apparent key component of long-term pancreas adaptation to increased insulin demand
publishDate 2004
url http://sedici.unlp.edu.ar/handle/10915/84370
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