Validation of a UV spectrophotometric method for determining trans-dehydrocrotonin in inclusion complexes with hydroxypropyl-β-cyclodextrin

Trans-dehydrocrotonin (t-DCTN) is a 19-nor-clerodan diterpen with several important pharmacological properties, including hypoglycemic and antitumor activity. However the low water solubility and hepatotoxicity of t-DCTN limit its use in therapeutic applications. Drug inclusion complexes with cyclod...

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Detalles Bibliográficos
Autores principales:	Santos Magalhães, Nereide S., Maciel, Maria A. M., Lira, Mariane C. B., Morais, Waldenice A., Lapenda, Taciana L.S.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2012
Materias:	Farmacia beta-Ciclodextrinas hydroxypropyl-β-cyclodextrin; inclusion complex; trans-dehydrocrotonin; UV spectrophotometry; validation Diterpenos de Clerodano Espectrometría
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8435 http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_15.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8435
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia beta-Ciclodextrinas hydroxypropyl-β-cyclodextrin; inclusion complex; trans-dehydrocrotonin; UV spectrophotometry; validation Diterpenos de Clerodano Espectrometría
spellingShingle	Farmacia beta-Ciclodextrinas hydroxypropyl-β-cyclodextrin; inclusion complex; trans-dehydrocrotonin; UV spectrophotometry; validation Diterpenos de Clerodano Espectrometría Santos Magalhães, Nereide S. Maciel, Maria A. M. Lira, Mariane C. B. Morais, Waldenice A. Lapenda, Taciana L.S. Validation of a UV spectrophotometric method for determining trans-dehydrocrotonin in inclusion complexes with hydroxypropyl-β-cyclodextrin
topic_facet	Farmacia beta-Ciclodextrinas hydroxypropyl-β-cyclodextrin; inclusion complex; trans-dehydrocrotonin; UV spectrophotometry; validation Diterpenos de Clerodano Espectrometría
description	Trans-dehydrocrotonin (t-DCTN) is a 19-nor-clerodan diterpen with several important pharmacological properties, including hypoglycemic and antitumor activity. However the low water solubility and hepatotoxicity of t-DCTN limit its use in therapeutic applications. Drug inclusion complexes with cyclodextrins (CDs) can modify physicochemical properties of parent drugs, such as improving their aqueous solubility and reducing their toxicity. A UV method was therefore validated for determining t-DCTN in HP-β-CD inclusion complexes with a view to future applications in research and therapy. The regression equation of the analytical curve (1–20 μg/mL) was [t-DCTN] = absorbance + 0.00147/0.04214. The precision of the method was satisfactory, producing values of relative standard deviation less than 2 % for all samples analyzed. The accuracy was between 99.6 and 100.02 %. The content of t-DCTN in tDCTN:HP-β-CD was 99.8 %. The UV validated method developed is straightforward and suitable for use in the routine analysis of t-DCTN complexed with hydroxypropyl-β-cyclodextrin.
format	Articulo Articulo
author	Santos Magalhães, Nereide S. Maciel, Maria A. M. Lira, Mariane C. B. Morais, Waldenice A. Lapenda, Taciana L.S.
author_facet	Santos Magalhães, Nereide S. Maciel, Maria A. M. Lira, Mariane C. B. Morais, Waldenice A. Lapenda, Taciana L.S.
author_sort	Santos Magalhães, Nereide S.
title	Validation of a UV spectrophotometric method for determining trans-dehydrocrotonin in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_short	Validation of a UV spectrophotometric method for determining trans-dehydrocrotonin in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_full	Validation of a UV spectrophotometric method for determining trans-dehydrocrotonin in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_fullStr	Validation of a UV spectrophotometric method for determining trans-dehydrocrotonin in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_full_unstemmed	Validation of a UV spectrophotometric method for determining trans-dehydrocrotonin in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_sort	validation of a uv spectrophotometric method for determining trans-dehydrocrotonin in inclusion complexes with hydroxypropyl-β-cyclodextrin
publishDate	2012
url	http://sedici.unlp.edu.ar/handle/10915/8435 http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_15.pdf
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Validation of a UV spectrophotometric method for determining trans-dehydrocrotonin in inclusion complexes with hydroxypropyl-β-cyclodextrin

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