Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction

Two DNA fragments, a 730-bp and a 900-bp fragment, one homologous to host cultivar specificity genes <i>nolBT</i> of <i>Sinorhizobium fredii</i> and the other one homologous to RSα, an insertion-like sequence present in <i>Bradyrhizobium japonicum</i>, were genera...

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Autores principales: Pastorino, Graciela Noemí, Martínez Alcántara, Virginia, Balatti, Pedro Alberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Ciencias Agrarias
Bradyrhizobium
Molecular marker
Polymerase chain reaction
Rhizobium
Sinorhizobium
Soybean
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84343
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84343
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Bradyrhizobium
Molecular marker
Polymerase chain reaction
Rhizobium
Sinorhizobium
Soybean
spellingShingle Ciencias Agrarias
Bradyrhizobium
Molecular marker
Polymerase chain reaction
Rhizobium
Sinorhizobium
Soybean
Pastorino, Graciela Noemí
Martínez Alcántara, Virginia
Balatti, Pedro Alberto
Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction
topic_facet Ciencias Agrarias
Bradyrhizobium
Molecular marker
Polymerase chain reaction
Rhizobium
Sinorhizobium
Soybean
description Two DNA fragments, a 730-bp and a 900-bp fragment, one homologous to host cultivar specificity genes <i>nolBT</i> of <i>Sinorhizobium fredii</i> and the other one homologous to RSα, an insertion-like sequence present in <i>Bradyrhizobium japonicum</i>, were generated by polymerase chain reaction (PCR) with two pairs of primers. The amount of each fragment generated by the multiplex PCR was proportional to the amount of template DNA present. The amplification of the 900-bp RSα fragment was more sensitive, since it was amplified from a smaller amount of template DNA than the 730-bp <i>nolBT</i> fragment. By running the multiplex reaction in the presence of template DNA isolated from different sources, we confirmed that the reaction can discriminate between <i>S. fredii</i>, <i>Bradyrhizobium japonicum</i> and <i>Sinorhizobium xinjiangensis</i>.
format Articulo
Articulo
author Pastorino, Graciela Noemí
Martínez Alcántara, Virginia
Balatti, Pedro Alberto
author_facet Pastorino, Graciela Noemí
Martínez Alcántara, Virginia
Balatti, Pedro Alberto
author_sort Pastorino, Graciela Noemí
title Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction
title_short Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction
title_full Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction
title_fullStr Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction
title_full_unstemmed Identification of fast and slow growing rhizobia nodulating soybean (Glycine max [L.] Merr) by a multiplex PCR reaction
title_sort identification of fast and slow growing rhizobia nodulating soybean (glycine max [l.] merr) by a multiplex pcr reaction
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/84343
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AT martinezalcantaravirginia identificationoffastandslowgrowingrhizobianodulatingsoybeanglycinemaxlmerrbyamultiplexpcrreaction
AT balattipedroalberto identificationoffastandslowgrowingrhizobianodulatingsoybeanglycinemaxlmerrbyamultiplexpcrreaction
bdutipo_str Repositorios
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