Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos

Translation is a sensitive regulatory step during cellular stress and the apoptosis response. Under such conditions, cap-dependent translation is reduced and internal ribosome entry site (IRES)-dependent translation plays a major role. However, many aspects of how mRNAs are translated under stress r...

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Autores principales: Hernández, Greco, Vázquez Pianzola, Paula, Sierra, José M., Rivera Pomar, Rolando Víctor
Formato: Articulo
Lenguaje:Inglés
Publicado: 2004
Materias:
Ciencias Exactas
1(3)67Af
Drosophila
eIF4E
hsp70
IRES-dependent translation
Reaper
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84317
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84317
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
1(3)67Af
Drosophila
eIF4E
hsp70
IRES-dependent translation
Reaper
spellingShingle Ciencias Exactas
1(3)67Af
Drosophila
eIF4E
hsp70
IRES-dependent translation
Reaper
Hernández, Greco
Vázquez Pianzola, Paula
Sierra, José M.
Rivera Pomar, Rolando Víctor
Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
topic_facet Ciencias Exactas
1(3)67Af
Drosophila
eIF4E
hsp70
IRES-dependent translation
Reaper
description Translation is a sensitive regulatory step during cellular stress and the apoptosis response. Under such conditions, cap-dependent translation is reduced and internal ribosome entry site (IRES)-dependent translation plays a major role. However, many aspects of how mRNAs are translated under stress remain to be elucidated. Here we report that <i>reaper</i> mRNA, a pro-apoptotic gene from <i>Drosophila melanogaster</i>, is translated in a cap-independent manner. In <i>Drosophila</i> mutant embryos devoid of the eukaryotic initiation factor 4E (eIF4E), <i>reaper</i> transcription is induced and apoptosis proceeds. In vitro translation experiments using wild-type and <i>eIF4E</i> mutant embryonic extracts show that reporter mRNA bearing <i>reaper</i> 5′ untranslated region (UTR) is effectively translated in a cap-independent manner. The 5′UTR of <i>reaper</i> exhibits a high degree of similarity with that of <i>Drosophila</i> <i>heat shock protein 70</i> mRNA, and both display IRES activity. Studies of mRNA association to polysomes in embryos indicate that both <i>reaper</i> and <i>heat shock protein 70</i> 70 mRNAs are recruited to polysomes under apoptosis or thermal stress. Our data suggest that <i>heat shock protein 70</i> 70 and <i>reaper</i>, two antagonizing factors in apoptosis, use a similar mechanism for protein synthesis.
format Articulo
Articulo
author Hernández, Greco
Vázquez Pianzola, Paula
Sierra, José M.
Rivera Pomar, Rolando Víctor
author_facet Hernández, Greco
Vázquez Pianzola, Paula
Sierra, José M.
Rivera Pomar, Rolando Víctor
author_sort Hernández, Greco
title Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
title_short Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
title_full Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
title_fullStr Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
title_full_unstemmed Internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mRNAs in Drosophila embryos
title_sort internal ribosome entry site drives cap-independent translation of reaper and heat shock protein 70 mrnas in drosophila embryos
publishDate 2004
url http://sedici.unlp.edu.ar/handle/10915/84317
work_keys_str_mv AT hernandezgreco internalribosomeentrysitedrivescapindependenttranslationofreaperandheatshockprotein70mrnasindrosophilaembryos
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AT sierrajosem internalribosomeentrysitedrivescapindependenttranslationofreaperandheatshockprotein70mrnasindrosophilaembryos
AT riverapomarrolandovictor internalribosomeentrysitedrivescapindependenttranslationofreaperandheatshockprotein70mrnasindrosophilaembryos
bdutipo_str Repositorios
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