Determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones

Anthraquinones-rich extracts of Heterophyllaea pustulata Hook f. (Rubiaceae) exhibited in vitro antiviral activity against Herpes Simplex Virus Type I, from which several anthraquinones (AQs) were isolated and identified. The Maximum Non-Cytotoxic Concentration (MNCC), the subtoxic concentration (Su...

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Autores principales:	Núñez Montoya, Susana C., Contigiani, Marta S., Marioni, Juliana, Aguilar, Juan J., Grasso, Sergio, Comini, Laura Raquel, Konigheim, Brenda S.
Formato:	Articulo Comunicacion
Lenguaje:	Inglés
Publicado:	2012
Materias:	Farmacia Toxicidad Células Vero Superóxidos Antraquinonas
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8428 http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_8.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

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spelling	I19-R120-10915-84282023-04-26T18:58:33Z http://sedici.unlp.edu.ar/handle/10915/8428 http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_8.pdf Determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones Núñez Montoya, Susana C. Contigiani, Marta S. Marioni, Juliana Aguilar, Juan J. Grasso, Sergio Comini, Laura Raquel Konigheim, Brenda S. 2012 2012-03-28T03:00:00Z en Farmacia Toxicidad Células Vero Superóxidos Antraquinonas Anthraquinones-rich extracts of Heterophyllaea pustulata Hook f. (Rubiaceae) exhibited in vitro antiviral activity against Herpes Simplex Virus Type I, from which several anthraquinones (AQs) were isolated and identified. The Maximum Non-Cytotoxic Concentration (MNCC), the subtoxic concentration (SubTC), and the CC50 of each AQ were determined on a mammalian eukaryotic cell line (Vero cells) by means of Neutral Red uptake assay; the cytopathic effect was simultaneously evaluated by optical microscopy. The range of concentrations where each AQ did not exhibit cytotoxicity was established, which is limited by the MNCC: rubiadin 1-methyl ether, damnacanthol and pustuline were found to be markedly less cytotoxic. To the remaining AQs, we could estimate a SubTC (about 10 μg/mL) that assures 80 % cellular viability. Therefore, we determined a concentration range which could be used to evaluate the antiviral effect of each AQ since it ensures the viability of the host cell. Colegio de Farmacéuticos de la Provincia de Buenos Aires Articulo Comunicacion http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 51-56
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia Toxicidad Células Vero Superóxidos Antraquinonas
spellingShingle	Farmacia Toxicidad Células Vero Superóxidos Antraquinonas Núñez Montoya, Susana C. Contigiani, Marta S. Marioni, Juliana Aguilar, Juan J. Grasso, Sergio Comini, Laura Raquel Konigheim, Brenda S. Determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones
topic_facet	Farmacia Toxicidad Células Vero Superóxidos Antraquinonas
description	Anthraquinones-rich extracts of Heterophyllaea pustulata Hook f. (Rubiaceae) exhibited in vitro antiviral activity against Herpes Simplex Virus Type I, from which several anthraquinones (AQs) were isolated and identified. The Maximum Non-Cytotoxic Concentration (MNCC), the subtoxic concentration (SubTC), and the CC50 of each AQ were determined on a mammalian eukaryotic cell line (Vero cells) by means of Neutral Red uptake assay; the cytopathic effect was simultaneously evaluated by optical microscopy. The range of concentrations where each AQ did not exhibit cytotoxicity was established, which is limited by the MNCC: rubiadin 1-methyl ether, damnacanthol and pustuline were found to be markedly less cytotoxic. To the remaining AQs, we could estimate a SubTC (about 10 μg/mL) that assures 80 % cellular viability. Therefore, we determined a concentration range which could be used to evaluate the antiviral effect of each AQ since it ensures the viability of the host cell.
format	Articulo Comunicacion
author	Núñez Montoya, Susana C. Contigiani, Marta S. Marioni, Juliana Aguilar, Juan J. Grasso, Sergio Comini, Laura Raquel Konigheim, Brenda S.
author_facet	Núñez Montoya, Susana C. Contigiani, Marta S. Marioni, Juliana Aguilar, Juan J. Grasso, Sergio Comini, Laura Raquel Konigheim, Brenda S.
author_sort	Núñez Montoya, Susana C.
title	Determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones
title_short	Determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones
title_full	Determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones
title_fullStr	Determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones
title_full_unstemmed	Determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones
title_sort	determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones
publishDate	2012
url	http://sedici.unlp.edu.ar/handle/10915/8428 http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_8.pdf
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Determination of non-toxic and subtoxic concentrations of potential antiviral natural anthraquinones

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