Mechanisms involved in the induction of aneuploidy: The significance of chromosome loss

The induction of aneuploidy by physical and chemical agents using different test systems was evaluated. The effect of X-rays, caffeine, acetaldehyde, ethanol, diethylstilbestrol, propionaldehyde, and chloral hydrate was studied by chromosome counting in Chinese hamster embryonic diploid cells. Aneug...

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Autores principales: Seoane, Analía Isabel, Güerci, Alba Mabel, Dulout, Fernando Noel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Veterinarias
induction of aneuploidy
chromosome loss
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84279
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84279
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
induction of aneuploidy
chromosome loss
spellingShingle Ciencias Veterinarias
induction of aneuploidy
chromosome loss
Seoane, Analía Isabel
Güerci, Alba Mabel
Dulout, Fernando Noel
Mechanisms involved in the induction of aneuploidy: The significance of chromosome loss
topic_facet Ciencias Veterinarias
induction of aneuploidy
chromosome loss
description The induction of aneuploidy by physical and chemical agents using different test systems was evaluated. The effect of X-rays, caffeine, acetaldehyde, ethanol, diethylstilbestrol, propionaldehyde, and chloral hydrate was studied by chromosome counting in Chinese hamster embryonic diploid cells. Aneugenic ability of cadmium chloride, cadmium sulfate, potassium dichromate, chromium chloride, nickel chloride, and nickel sulfate was assessed by means of anaphase-telophase analysis in Chinese hamster ovary cells. Chromosome counting in human fibroblasts (MRC-5 cell line) was employed to evaluate the effect of cacodilic acid, cadmium chloride, cadmium sulfate, and potassium dichromate. Finally, the induction of kinetochore-positive and kinetochore negative micronuclei by cadmium chloride, cadmium sulfate, potassium dichromate, chromium chloride, and nickel chloride was studied using CREST antibodies. When the effect of different agents was determined by chromosome counting, an increase of hypoploid but not of hyperploid cells was observed. Anaphase-telophase analysis showed that metal salts increased the frequency of lagging chromosomes. This finding has been confirmed by the increment of kinetochore-positive micronuclei using CREST antibodies. Therefore, chromosome loss could be considered as the main cause of induced aneuploidy.
format Articulo
Articulo
author Seoane, Analía Isabel
Güerci, Alba Mabel
Dulout, Fernando Noel
author_facet Seoane, Analía Isabel
Güerci, Alba Mabel
Dulout, Fernando Noel
author_sort Seoane, Analía Isabel
title Mechanisms involved in the induction of aneuploidy: The significance of chromosome loss
title_short Mechanisms involved in the induction of aneuploidy: The significance of chromosome loss
title_full Mechanisms involved in the induction of aneuploidy: The significance of chromosome loss
title_fullStr Mechanisms involved in the induction of aneuploidy: The significance of chromosome loss
title_full_unstemmed Mechanisms involved in the induction of aneuploidy: The significance of chromosome loss
title_sort mechanisms involved in the induction of aneuploidy: the significance of chromosome loss
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/84279
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