Anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid Mk from Ganoderma lucidum mycelia in cervical cancer HeLa cells
Ganoderic acid Mk (GA-Mk), a triterpenoid acid, was isolated from the mycelia of Ganoderma lucidum, and no biological activity of GA-Mk has ever been reported. In this work, we investigated the effect of GA-Mk on the cell proliferation and apoptosis in HeLa cells. The MTT results demonstrated that G...
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| Autores principales: | , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2012
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8427 http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_7.pdf |
| Aporte de: |
| Sumario: | Ganoderic acid Mk (GA-Mk), a triterpenoid acid, was isolated from the mycelia of Ganoderma lucidum, and no biological activity of GA-Mk has ever been reported. In this work, we investigated the effect of GA-Mk on the cell proliferation and apoptosis in HeLa cells. The MTT results demonstrated that GA-Mk displayed interesting cytotoxicity toward various human cancer cell lines. Bromodeoxyuridine (BrdU) incorporation assay showed that GA-Mk had a dose-dependent inhibitory effect on proliferation of HeLa cells. The flow cytometry analysis indicated that the treatment of HeLa cells with GA-Mk increased the rate of early and late apoptotic cells in a dose-dependent manner. Furthermore, GA-Mk inducing apoptosis was in association with the burst of intracellular reactive oxygen species (ROS), the decrease of the mitochondrial membrane potential and the increase of caspase-3 and caspase-9 activities. These results demonstrated that GA-Mk was efficiently anti-proliferative and could induce apoptosis of HeLa cells by mitochondria-mediated pathway, and it may serve as a promising candidate for the treatment of cervical cancer. |
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