Anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid Mk from Ganoderma lucidum mycelia in cervical cancer HeLa cells

Ganoderic acid Mk (GA-Mk), a triterpenoid acid, was isolated from the mycelia of Ganoderma lucidum, and no biological activity of GA-Mk has ever been reported. In this work, we investigated the effect of GA-Mk on the cell proliferation and apoptosis in HeLa cells. The MTT results demonstrated that G...

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Detalles Bibliográficos
Autores principales: Zhong, Jian-Jiang, Li, Ying-Bo, Liu, Ru-Ming
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Ganoderma
anti-proliferation; ganoderic acid Mk (GA-Mk)
Triterpenos
Mitocondrias
Células HeLa
Apoptosis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8427
http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_7.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8427
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Ganoderma
anti-proliferation; ganoderic acid Mk (GA-Mk)
Triterpenos
Mitocondrias
Células HeLa
Apoptosis
spellingShingle Farmacia
Ganoderma
anti-proliferation; ganoderic acid Mk (GA-Mk)
Triterpenos
Mitocondrias
Células HeLa
Apoptosis
Zhong, Jian-Jiang
Li, Ying-Bo
Liu, Ru-Ming
Anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid Mk from Ganoderma lucidum mycelia in cervical cancer HeLa cells
topic_facet Farmacia
Ganoderma
anti-proliferation; ganoderic acid Mk (GA-Mk)
Triterpenos
Mitocondrias
Células HeLa
Apoptosis
description Ganoderic acid Mk (GA-Mk), a triterpenoid acid, was isolated from the mycelia of Ganoderma lucidum, and no biological activity of GA-Mk has ever been reported. In this work, we investigated the effect of GA-Mk on the cell proliferation and apoptosis in HeLa cells. The MTT results demonstrated that GA-Mk displayed interesting cytotoxicity toward various human cancer cell lines. Bromodeoxyuridine (BrdU) incorporation assay showed that GA-Mk had a dose-dependent inhibitory effect on proliferation of HeLa cells. The flow cytometry analysis indicated that the treatment of HeLa cells with GA-Mk increased the rate of early and late apoptotic cells in a dose-dependent manner. Furthermore, GA-Mk inducing apoptosis was in association with the burst of intracellular reactive oxygen species (ROS), the decrease of the mitochondrial membrane potential and the increase of caspase-3 and caspase-9 activities. These results demonstrated that GA-Mk was efficiently anti-proliferative and could induce apoptosis of HeLa cells by mitochondria-mediated pathway, and it may serve as a promising candidate for the treatment of cervical cancer.
format Articulo
Articulo
author Zhong, Jian-Jiang
Li, Ying-Bo
Liu, Ru-Ming
author_facet Zhong, Jian-Jiang
Li, Ying-Bo
Liu, Ru-Ming
author_sort Zhong, Jian-Jiang
title Anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid Mk from Ganoderma lucidum mycelia in cervical cancer HeLa cells
title_short Anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid Mk from Ganoderma lucidum mycelia in cervical cancer HeLa cells
title_full Anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid Mk from Ganoderma lucidum mycelia in cervical cancer HeLa cells
title_fullStr Anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid Mk from Ganoderma lucidum mycelia in cervical cancer HeLa cells
title_full_unstemmed Anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid Mk from Ganoderma lucidum mycelia in cervical cancer HeLa cells
title_sort anti-proliferation and induced mitochondria-mediated apoptosis of ganoderic acid mk from ganoderma lucidum mycelia in cervical cancer hela cells
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/8427
http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_7.pdf
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AT liyingbo antiproliferationandinducedmitochondriamediatedapoptosisofganodericacidmkfromganodermalucidummyceliaincervicalcancerhelacells
AT liuruming antiproliferationandinducedmitochondriamediatedapoptosisofganodericacidmkfromganodermalucidummyceliaincervicalcancerhelacells
bdutipo_str Repositorios
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