Host genes involved in nodulation preference in common bean (Phaseolus vulgaris)-Rhizobium etli symbiosis revealed by suppressive subtractive hybridization

Common bean cultivars are nodulated preferentially by <i>Rhizobium etli</i> lineages from the same center of host diversification. Nodulation was found to be earlier and numerous in bean plants inoculated with the cognate strain. We predicted that analysis of transcripts at early stages...

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Autores principales: Peltzer Meschini, Eitel, Blanco, Flavio Antonio, Zanetti, María Eugenia, Beker, María Pía, Küster, Helge, Pühler, Alfred, Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Ciencias Exactas
Legume
Root hair
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84250
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84250
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Legume
Root hair
spellingShingle Ciencias Exactas
Legume
Root hair
Peltzer Meschini, Eitel
Blanco, Flavio Antonio
Zanetti, María Eugenia
Beker, María Pía
Küster, Helge
Pühler, Alfred
Aguilar, Orlando Mario
Host genes involved in nodulation preference in common bean (Phaseolus vulgaris)-Rhizobium etli symbiosis revealed by suppressive subtractive hybridization
topic_facet Ciencias Exactas
Legume
Root hair
description Common bean cultivars are nodulated preferentially by <i>Rhizobium etli</i> lineages from the same center of host diversification. Nodulation was found to be earlier and numerous in bean plants inoculated with the cognate strain. We predicted that analysis of transcripts at early stages of the interaction between host and rhizobium would identify plant genes that are most likely to be involved in this preferential nodulation. Therefore, we applied a suppressive subtractive hybridization approach in which cDNA from a Mesoamerican cultivar inoculated with either the more- or less-efficient strain of <i>R. etli</i> was used as the driver and the tester, respectively. Forty-one independent tentative consensus sequences (TCs) were obtained and classified into different functional categories. Of 11 selected TCs, 9 were confirmed by quantitative reverse-transcriptase polymerase chain reaction. Two genes show high homology to previously characterized plant receptors. Two other upregulated genes encode for Rab11, a member of the small GTP-binding protein family, and HAP5, a subunit of the heterotrimeric CCAAT-transcription factor. Interestingly, one of the TCs encodes for an isoflavone reductase, which may lead to earlier Nod factor production by specific strains of rhizobia. The transcript abundance of selected cDNAs also was found to be higher in mature nodules of the more efficient interaction. Small or no differences were observed when an Andean bean cultivar was inoculated with a cognate strain, suggesting involvement of these genes in the strain-specific response. The potential role of these genes in the early preferential symbiotic interaction is discussed.
format Articulo
Articulo
author Peltzer Meschini, Eitel
Blanco, Flavio Antonio
Zanetti, María Eugenia
Beker, María Pía
Küster, Helge
Pühler, Alfred
Aguilar, Orlando Mario
author_facet Peltzer Meschini, Eitel
Blanco, Flavio Antonio
Zanetti, María Eugenia
Beker, María Pía
Küster, Helge
Pühler, Alfred
Aguilar, Orlando Mario
author_sort Peltzer Meschini, Eitel
title Host genes involved in nodulation preference in common bean (Phaseolus vulgaris)-Rhizobium etli symbiosis revealed by suppressive subtractive hybridization
title_short Host genes involved in nodulation preference in common bean (Phaseolus vulgaris)-Rhizobium etli symbiosis revealed by suppressive subtractive hybridization
title_full Host genes involved in nodulation preference in common bean (Phaseolus vulgaris)-Rhizobium etli symbiosis revealed by suppressive subtractive hybridization
title_fullStr Host genes involved in nodulation preference in common bean (Phaseolus vulgaris)-Rhizobium etli symbiosis revealed by suppressive subtractive hybridization
title_full_unstemmed Host genes involved in nodulation preference in common bean (Phaseolus vulgaris)-Rhizobium etli symbiosis revealed by suppressive subtractive hybridization
title_sort host genes involved in nodulation preference in common bean (phaseolus vulgaris)-rhizobium etli symbiosis revealed by suppressive subtractive hybridization
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/84250
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