Granulosain I, a cysteine protease isolated from ripe fruits of Solanum granuloso-leprosum (Solanaceae)

A new cysteine peptidase (Granulosain I) was isolated from ripe fruits of <i>Solanum granuloso-leprosum</i> Dunal (Solanaceae) by means of precipitation with organic solvent and cation exchange chromatography. The enzyme showed a single band by SDS-PAGE, its molecular mass was 24,746 Da...

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Autores principales: Vallés, Diego, Bruno, Mariela Anahí, López, Laura María Isabel, Caffini, Néstor Oscar, Cantera, Ana María B.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Ciencias Exactas
Cysteine protease
Plant peptidases
Solanaceae
Solanum granuloso-leprosum
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84246
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84246
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Cysteine protease
Plant peptidases
Solanaceae
Solanum granuloso-leprosum
spellingShingle Ciencias Exactas
Cysteine protease
Plant peptidases
Solanaceae
Solanum granuloso-leprosum
Vallés, Diego
Bruno, Mariela Anahí
López, Laura María Isabel
Caffini, Néstor Oscar
Cantera, Ana María B.
Granulosain I, a cysteine protease isolated from ripe fruits of Solanum granuloso-leprosum (Solanaceae)
topic_facet Ciencias Exactas
Cysteine protease
Plant peptidases
Solanaceae
Solanum granuloso-leprosum
description A new cysteine peptidase (Granulosain I) was isolated from ripe fruits of <i>Solanum granuloso-leprosum</i> Dunal (Solanaceae) by means of precipitation with organic solvent and cation exchange chromatography. The enzyme showed a single band by SDS-PAGE, its molecular mass was 24,746 Da (MALDI-TOF/MS) and its isoelectric point was higher than 9.3. It showed maximum activity (more than 90%) in the pH range 7-8.6. Granulosain I was completely inhibited by E-64 and activated by the addition of cysteine or 2-mercaptoethanol, confirming its cysteinic nature. The kinetic studies carried out with PFLNA as substrate, showed an affinity (Km 0.6 mM) slightly lower than those of other known plant cysteine proteases (papain and bromelain). The N-terminal sequence of granulosain I (DRLPASVDWRGKGVLVLVKNQGQC) exhibited a close homology with other cysteine proteases belonging to the C1A family.
format Articulo
Articulo
author Vallés, Diego
Bruno, Mariela Anahí
López, Laura María Isabel
Caffini, Néstor Oscar
Cantera, Ana María B.
author_facet Vallés, Diego
Bruno, Mariela Anahí
López, Laura María Isabel
Caffini, Néstor Oscar
Cantera, Ana María B.
author_sort Vallés, Diego
title Granulosain I, a cysteine protease isolated from ripe fruits of Solanum granuloso-leprosum (Solanaceae)
title_short Granulosain I, a cysteine protease isolated from ripe fruits of Solanum granuloso-leprosum (Solanaceae)
title_full Granulosain I, a cysteine protease isolated from ripe fruits of Solanum granuloso-leprosum (Solanaceae)
title_fullStr Granulosain I, a cysteine protease isolated from ripe fruits of Solanum granuloso-leprosum (Solanaceae)
title_full_unstemmed Granulosain I, a cysteine protease isolated from ripe fruits of Solanum granuloso-leprosum (Solanaceae)
title_sort granulosain i, a cysteine protease isolated from ripe fruits of solanum granuloso-leprosum (solanaceae)
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/84246
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bdutipo_str Repositorios
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