Development and validation of LC-ESI-MS method for the quantification of dapoxetine in rat plasma

A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of dapoxetine in rat plasma using one-step protein precipitation was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile...

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Autores principales: Ma, Jianshe, Luo, Shunbin, Chen, Chan, Wang, Jinjin, Wang, Zhibin, Wang, Zhiyi
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Serotonina
Espectrometría de Masas
Cromatografía Liquida
Plasma
Ratas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8421
http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_1.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8421
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Serotonina
Espectrometría de Masas
Cromatografía Liquida
Plasma
Ratas
spellingShingle Farmacia
Serotonina
Espectrometría de Masas
Cromatografía Liquida
Plasma
Ratas
Ma, Jianshe
Luo, Shunbin
Chen, Chan
Wang, Jinjin
Wang, Zhibin
Wang, Zhiyi
Development and validation of LC-ESI-MS method for the quantification of dapoxetine in rat plasma
topic_facet Farmacia
Serotonina
Espectrometría de Masas
Cromatografía Liquida
Plasma
Ratas
description A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of dapoxetine in rat plasma using one-step protein precipitation was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used in sample preparation. Chromatographically separation was achieved on an SB-C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1 % formic acid as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 306 for dapoxetine and m/z 326 for the IS. Calibration plots were linear over the range of 5-1000 ng/mL for dapoxetine in rat plasma. Lower limit of quantification (LLOQ) for dapoxetine was 5 ng/mL. Mean recovery of dapoxetine from plasma was in the range of 92.4-98.6 %. CV of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of dapoxetine in rat plasma.
format Articulo
Articulo
author Ma, Jianshe
Luo, Shunbin
Chen, Chan
Wang, Jinjin
Wang, Zhibin
Wang, Zhiyi
author_facet Ma, Jianshe
Luo, Shunbin
Chen, Chan
Wang, Jinjin
Wang, Zhibin
Wang, Zhiyi
author_sort Ma, Jianshe
title Development and validation of LC-ESI-MS method for the quantification of dapoxetine in rat plasma
title_short Development and validation of LC-ESI-MS method for the quantification of dapoxetine in rat plasma
title_full Development and validation of LC-ESI-MS method for the quantification of dapoxetine in rat plasma
title_fullStr Development and validation of LC-ESI-MS method for the quantification of dapoxetine in rat plasma
title_full_unstemmed Development and validation of LC-ESI-MS method for the quantification of dapoxetine in rat plasma
title_sort development and validation of lc-esi-ms method for the quantification of dapoxetine in rat plasma
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/8421
http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_1.pdf
work_keys_str_mv AT majianshe developmentandvalidationoflcesimsmethodforthequantificationofdapoxetineinratplasma
AT luoshunbin developmentandvalidationoflcesimsmethodforthequantificationofdapoxetineinratplasma
AT chenchan developmentandvalidationoflcesimsmethodforthequantificationofdapoxetineinratplasma
AT wangjinjin developmentandvalidationoflcesimsmethodforthequantificationofdapoxetineinratplasma
AT wangzhibin developmentandvalidationoflcesimsmethodforthequantificationofdapoxetineinratplasma
AT wangzhiyi developmentandvalidationoflcesimsmethodforthequantificationofdapoxetineinratplasma
bdutipo_str Repositorios
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