Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in <i>Bordetella pertussis</i>, the agent responsible for whooping cough. Recent reports indicate that <i>B. pertussis</i> adopts a sessile biofilm as...
Guardado en:
| Autores principales: | , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/84205 |
| Aporte de: |
| Sumario: | Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in <i>Bordetella pertussis</i>, the agent responsible for whooping cough. Recent reports indicate that <i>B. pertussis</i> adopts a sessile biofilm as a strategy to persistently colonize the human host. However, since research in the past mainly focused on the planktonic lifestyle of <i>B. pertussis</i>, knowledge on biofilm formation of this important human pathogen is still limited. Comparative studies were carried out by combining 2-DE and Fourier transform infrared (FT-IR) spectroscopy with multivariate statistical methods. These complementary approaches demonstrated that biofilm development has a distinctive impact on <i>B. pertussis</i> physiology. Results from MALDI-TOF/MS identification of proteins together with results from FT-IR spectroscopy revealed the biosynthesis of a putative acidic-type polysaccharide polymer as the most distinctive trait of <i>B. pertussis</i> life in a biofilm. Additionally, expression of proteins known to be involved in cellular regulatory circuits, cell attachment and virulence was altered in sessile cells, which strongly suggests a significant impact of biofilm development on <i>B. pertussis</i> pathogenesis. In summary, our work showed that the combination of proteomics and FT-IR spectroscopy with multivariate statistical analysis provides a powerful tool to gain further insight into bacterial lifestyles. |
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