Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis

Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in <i>Bordetella pertussis</i>, the agent responsible for whooping cough. Recent reports indicate that <i>B. pertussis</i> adopts a sessile biofilm as...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Serra, Diego Omar, Lücking, Genia, Weiland, Florian, Schulz, Stefan, Görg, Angelika, Yantorno, Osvaldo Miguel, Ehling Schulz, Monika
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Ciencias Exactas
Biofilm
Bordetella pertussis
FT-IR spectroscopy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84205
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84205
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biofilm
Bordetella pertussis
FT-IR spectroscopy
spellingShingle Ciencias Exactas
Biofilm
Bordetella pertussis
FT-IR spectroscopy
Serra, Diego Omar
Lücking, Genia
Weiland, Florian
Schulz, Stefan
Görg, Angelika
Yantorno, Osvaldo Miguel
Ehling Schulz, Monika
Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
topic_facet Ciencias Exactas
Biofilm
Bordetella pertussis
FT-IR spectroscopy
description Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in <i>Bordetella pertussis</i>, the agent responsible for whooping cough. Recent reports indicate that <i>B. pertussis</i> adopts a sessile biofilm as a strategy to persistently colonize the human host. However, since research in the past mainly focused on the planktonic lifestyle of <i>B. pertussis</i>, knowledge on biofilm formation of this important human pathogen is still limited. Comparative studies were carried out by combining 2-DE and Fourier transform infrared (FT-IR) spectroscopy with multivariate statistical methods. These complementary approaches demonstrated that biofilm development has a distinctive impact on <i>B. pertussis</i> physiology. Results from MALDI-TOF/MS identification of proteins together with results from FT-IR spectroscopy revealed the biosynthesis of a putative acidic-type polysaccharide polymer as the most distinctive trait of <i>B. pertussis</i> life in a biofilm. Additionally, expression of proteins known to be involved in cellular regulatory circuits, cell attachment and virulence was altered in sessile cells, which strongly suggests a significant impact of biofilm development on <i>B. pertussis</i> pathogenesis. In summary, our work showed that the combination of proteomics and FT-IR spectroscopy with multivariate statistical analysis provides a powerful tool to gain further insight into bacterial lifestyles.
format Articulo
Articulo
author Serra, Diego Omar
Lücking, Genia
Weiland, Florian
Schulz, Stefan
Görg, Angelika
Yantorno, Osvaldo Miguel
Ehling Schulz, Monika
author_facet Serra, Diego Omar
Lücking, Genia
Weiland, Florian
Schulz, Stefan
Görg, Angelika
Yantorno, Osvaldo Miguel
Ehling Schulz, Monika
author_sort Serra, Diego Omar
title Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title_short Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title_full Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title_fullStr Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title_full_unstemmed Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis
title_sort proteome approaches combined with fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in bordetella pertussis
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/84205
work_keys_str_mv AT serradiegoomar proteomeapproachescombinedwithfouriertransforminfraredspectroscopyrevealedadistinctivebiofilmphysiologyinbordetellapertussis
AT luckinggenia proteomeapproachescombinedwithfouriertransforminfraredspectroscopyrevealedadistinctivebiofilmphysiologyinbordetellapertussis
AT weilandflorian proteomeapproachescombinedwithfouriertransforminfraredspectroscopyrevealedadistinctivebiofilmphysiologyinbordetellapertussis
AT schulzstefan proteomeapproachescombinedwithfouriertransforminfraredspectroscopyrevealedadistinctivebiofilmphysiologyinbordetellapertussis
AT gorgangelika proteomeapproachescombinedwithfouriertransforminfraredspectroscopyrevealedadistinctivebiofilmphysiologyinbordetellapertussis
AT yantornoosvaldomiguel proteomeapproachescombinedwithfouriertransforminfraredspectroscopyrevealedadistinctivebiofilmphysiologyinbordetellapertussis
AT ehlingschulzmonika proteomeapproachescombinedwithfouriertransforminfraredspectroscopyrevealedadistinctivebiofilmphysiologyinbordetellapertussis
bdutipo_str Repositorios
_version_ 1764820488471707649

Ejemplares similares