Determination of ramosetron in rat plasma by LC-ESI-MS and its application

A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of ramosetron in rat plasma using one-step protein precipitation was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile...

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Autores principales: Lin, Guanyang, Chen, Xiaole, Zheng, Meiqin, Wang, Youpei, Pang, Lingxia, Wang, Qing
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Plasma
Cromatografía Liquida
Espectrometría de Masas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8420
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_2_5.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8420
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Plasma
Cromatografía Liquida
Espectrometría de Masas
spellingShingle Farmacia
Plasma
Cromatografía Liquida
Espectrometría de Masas
Lin, Guanyang
Chen, Xiaole
Zheng, Meiqin
Wang, Youpei
Pang, Lingxia
Wang, Qing
Determination of ramosetron in rat plasma by LC-ESI-MS and its application
topic_facet Farmacia
Plasma
Cromatografía Liquida
Espectrometría de Masas
description A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of ramosetron in rat plasma using one-step protein precipitation was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographically separation was achieved on an SB-C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1 % formic acid as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 280 for ramosetron and m/z 326 for the IS. Calibration plots were linear over the range of 10-1000 ng/mL for ramosetron in rat plasma. Lower limit of quantification (LLOQ) for Ramosetron was 10 ng/mL. Mean recovery of ramosetron from plasma was in the range of 88.5-92.8 %. CV of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic study for determination of ramosetron in rat plasma.
format Articulo
Comunicacion
author Lin, Guanyang
Chen, Xiaole
Zheng, Meiqin
Wang, Youpei
Pang, Lingxia
Wang, Qing
author_facet Lin, Guanyang
Chen, Xiaole
Zheng, Meiqin
Wang, Youpei
Pang, Lingxia
Wang, Qing
author_sort Lin, Guanyang
title Determination of ramosetron in rat plasma by LC-ESI-MS and its application
title_short Determination of ramosetron in rat plasma by LC-ESI-MS and its application
title_full Determination of ramosetron in rat plasma by LC-ESI-MS and its application
title_fullStr Determination of ramosetron in rat plasma by LC-ESI-MS and its application
title_full_unstemmed Determination of ramosetron in rat plasma by LC-ESI-MS and its application
title_sort determination of ramosetron in rat plasma by lc-esi-ms and its application
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8420
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_2_5.pdf
work_keys_str_mv AT linguanyang determinationoframosetroninratplasmabylcesimsanditsapplication
AT chenxiaole determinationoframosetroninratplasmabylcesimsanditsapplication
AT zhengmeiqin determinationoframosetroninratplasmabylcesimsanditsapplication
AT wangyoupei determinationoframosetroninratplasmabylcesimsanditsapplication
AT panglingxia determinationoframosetroninratplasmabylcesimsanditsapplication
AT wangqing determinationoframosetroninratplasmabylcesimsanditsapplication
bdutipo_str Repositorios
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