The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation

The segmentation process in insects depends on a hierarchical cascade of gene activity. The first effectors downstream of the maternal activation are the gap genes, which divide the embryo in broad fields. We discovered a sequence corresponding to the leucine-zipper domain of the orthologue of the g...

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Autores principales: Lavore, Andrés E., Pagola, Lucía Elena, Esponda Behrens, Natalia, Rivera Pomar, Rolando Víctor
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Exactas
Gap gene
Giant
Maternal expression
Regulatory cluster
Rhodnius
Segmentation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84164
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84164
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Gap gene
Giant
Maternal expression
Regulatory cluster
Rhodnius
Segmentation
spellingShingle Ciencias Exactas
Gap gene
Giant
Maternal expression
Regulatory cluster
Rhodnius
Segmentation
Lavore, Andrés E.
Pagola, Lucía Elena
Esponda Behrens, Natalia
Rivera Pomar, Rolando Víctor
The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
topic_facet Ciencias Exactas
Gap gene
Giant
Maternal expression
Regulatory cluster
Rhodnius
Segmentation
description The segmentation process in insects depends on a hierarchical cascade of gene activity. The first effectors downstream of the maternal activation are the gap genes, which divide the embryo in broad fields. We discovered a sequence corresponding to the leucine-zipper domain of the orthologue of the gene giant (Rp- gt) in traces from the genome of Rhodnius prolixus, a hemipteran with intermediate germ-band development. We cloned the Rp- gt gene from a normalized cDNA library and characterized its expression and function. Bioinformatic analysis of 12.5. kbp of genomic sequence containing the Rp- gt transcriptional unit shows a cluster of bona fide regulatory binding sites, which is similar in location and structure to the predicted posterior expression domain of the Drosophila orthologue. Rp-gt is expressed in ovaries and maternally supplied in the early embryo. The maternal contribution forms a gradient of scattered patches of mRNA in the preblastoderm embryo. Zygotic Rp-gt is expressed in two domains that after germ band extension are restricted to the head and the posterior growth zone. Parental RNAi shows that Rp-gt is required for proper head and abdomen formation. The head lacks mandibulary and maxillary appendages and shows reduced clypeus-labrum, while the abdomen lacks anterior segments. We conclude that Rp-gt is a gap gene on the head and abdomen and, in addition, has a function in patterning the anterior head capsule suggesting that the function of gt in hemipterans is more similar to dipterans than expected.
format Articulo
Articulo
author Lavore, Andrés E.
Pagola, Lucía Elena
Esponda Behrens, Natalia
Rivera Pomar, Rolando Víctor
author_facet Lavore, Andrés E.
Pagola, Lucía Elena
Esponda Behrens, Natalia
Rivera Pomar, Rolando Víctor
author_sort Lavore, Andrés E.
title The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title_short The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title_full The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title_fullStr The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title_full_unstemmed The gap gene giant of Rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
title_sort gap gene giant of rhodnius prolixus is maternally expressed and required for proper head and abdomen formation
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/84164
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