Fast determination of voriconazole in oral fluid using microextractionby packed sorbent and HPLC with fluorescence detection

A fast and reliable method for the determination of voriconazole in oral fluid using microextraction by packed sorbent and liquid chromatography with fluorescence detection was developed and validated. MEPS was performed at basic pH with only 50 μL of oral fluid and the extract was injected without...

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Autores principales: Antunes, Marina V., Linden, Rafael, Pasqualotto, Alessandro C., Andreolla, Huander F., Spaniol, Bárbara, Bordin, Natália A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Agentes Antifúngicos
Cromatografía
Monitoreo de Drogas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8414
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_26.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8414
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Agentes Antifúngicos
Cromatografía
Monitoreo de Drogas
spellingShingle Farmacia
Agentes Antifúngicos
Cromatografía
Monitoreo de Drogas
Antunes, Marina V.
Linden, Rafael
Pasqualotto, Alessandro C.
Andreolla, Huander F.
Spaniol, Bárbara
Bordin, Natália A.
Fast determination of voriconazole in oral fluid using microextractionby packed sorbent and HPLC with fluorescence detection
topic_facet Farmacia
Agentes Antifúngicos
Cromatografía
Monitoreo de Drogas
description A fast and reliable method for the determination of voriconazole in oral fluid using microextraction by packed sorbent and liquid chromatography with fluorescence detection was developed and validated. MEPS was performed at basic pH with only 50 μL of oral fluid and the extract was injected without an evaporation step. The overall procedure, including extraction and chromatographic analysis, took only 15 min. Voriconazole and internal standard were separated on a Lichrospher RP 8ec column (250 x 4 mm, particle diameter 5 μm) eluted with a mobile phase composed of phosphate pH 2.3 (containing 0.1 % triethylamine) and acetonitrile (64:36, v/v) at a flow rate of 1.4 mL min-1 . Total run time was 11 min, with detection being performed with excitation at 254 and emission at 372 nm. The method was successfully applied to oral fluid samples, with voriconazole concentrations presenting an average of 57.6 % of those measured in paired plasma samples.
format Articulo
Articulo
author Antunes, Marina V.
Linden, Rafael
Pasqualotto, Alessandro C.
Andreolla, Huander F.
Spaniol, Bárbara
Bordin, Natália A.
author_facet Antunes, Marina V.
Linden, Rafael
Pasqualotto, Alessandro C.
Andreolla, Huander F.
Spaniol, Bárbara
Bordin, Natália A.
author_sort Antunes, Marina V.
title Fast determination of voriconazole in oral fluid using microextractionby packed sorbent and HPLC with fluorescence detection
title_short Fast determination of voriconazole in oral fluid using microextractionby packed sorbent and HPLC with fluorescence detection
title_full Fast determination of voriconazole in oral fluid using microextractionby packed sorbent and HPLC with fluorescence detection
title_fullStr Fast determination of voriconazole in oral fluid using microextractionby packed sorbent and HPLC with fluorescence detection
title_full_unstemmed Fast determination of voriconazole in oral fluid using microextractionby packed sorbent and HPLC with fluorescence detection
title_sort fast determination of voriconazole in oral fluid using microextractionby packed sorbent and hplc with fluorescence detection
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8414
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_26.pdf
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