Zinc-binding properties of Junín virus nucleocapsid protein

The arenavirus nucleocapsid protein (N) is a highly basic 63 kDa protein with a dual function during the virus life-cycle. First, it is involved in essential steps of genome replication, promoting the synthesis of the full-length antigenomic copy of S RNA, and second it associates with the genomic R...

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Autores principales: Tortorici, María Alejandra, Ghiringhelli, P. D., Lozano, Mario E., Albariño, César G., Romanowski, Víctor
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Biología
Virus Junin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84101
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84101
record_format dspace
spelling I19-R120-10915-841012024-12-26T16:48:07Z http://sedici.unlp.edu.ar/handle/10915/84101 Zinc-binding properties of Junín virus nucleocapsid protein Tortorici, María Alejandra Ghiringhelli, P. D. Lozano, Mario E. Albariño, César G. Romanowski, Víctor 2001 2019-10-25T17:33:03Z en Biología Virus Junin The arenavirus nucleocapsid protein (N) is a highly basic 63 kDa protein with a dual function during the virus life-cycle. First, it is involved in essential steps of genome replication, promoting the synthesis of the full-length antigenomic copy of S RNA, and second it associates with the genomic RNA to form the nucleocapsid. We have expressed the N protein of Junín virus in E. coli and shown that it binds zinc in vitro. This property is in agreement with the presence in the carboxy-terminal region of the N protein of the CX2HX23CX4C sequence, which resembles a classical zinc-finger motif. The specificity for zinc binding was demonstrated by competition with other divalent metal ions. The ability of the predicted motif to bind zinc was established by analysis of a series of N mutants, including truncated variants and amino acid substitutions. In addition, alternative zinc-binding sites were found. Instituto de Biotecnologia y Biologia Molecular Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 121-128
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Virus Junin
spellingShingle Biología
Virus Junin
Tortorici, María Alejandra
Ghiringhelli, P. D.
Lozano, Mario E.
Albariño, César G.
Romanowski, Víctor
Zinc-binding properties of Junín virus nucleocapsid protein
topic_facet Biología
Virus Junin
description The arenavirus nucleocapsid protein (N) is a highly basic 63 kDa protein with a dual function during the virus life-cycle. First, it is involved in essential steps of genome replication, promoting the synthesis of the full-length antigenomic copy of S RNA, and second it associates with the genomic RNA to form the nucleocapsid. We have expressed the N protein of Junín virus in E. coli and shown that it binds zinc in vitro. This property is in agreement with the presence in the carboxy-terminal region of the N protein of the CX2HX23CX4C sequence, which resembles a classical zinc-finger motif. The specificity for zinc binding was demonstrated by competition with other divalent metal ions. The ability of the predicted motif to bind zinc was established by analysis of a series of N mutants, including truncated variants and amino acid substitutions. In addition, alternative zinc-binding sites were found.
format Articulo
Articulo
author Tortorici, María Alejandra
Ghiringhelli, P. D.
Lozano, Mario E.
Albariño, César G.
Romanowski, Víctor
author_facet Tortorici, María Alejandra
Ghiringhelli, P. D.
Lozano, Mario E.
Albariño, César G.
Romanowski, Víctor
author_sort Tortorici, María Alejandra
title Zinc-binding properties of Junín virus nucleocapsid protein
title_short Zinc-binding properties of Junín virus nucleocapsid protein
title_full Zinc-binding properties of Junín virus nucleocapsid protein
title_fullStr Zinc-binding properties of Junín virus nucleocapsid protein
title_full_unstemmed Zinc-binding properties of Junín virus nucleocapsid protein
title_sort zinc-binding properties of junín virus nucleocapsid protein
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/84101
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AT lozanomarioe zincbindingpropertiesofjuninvirusnucleocapsidprotein
AT albarinocesarg zincbindingpropertiesofjuninvirusnucleocapsidprotein
AT romanowskivictor zincbindingpropertiesofjuninvirusnucleocapsidprotein
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