Genotypic and phenotypic characterization of Bordetella pertussis strains used in different vaccine formulations in Latin America

Aim: To characterize <i>Bordetella pertussis</i> vaccine strains in comparison with current circulating bacteria. Methods and Results: Genomic and proteomic analyses of <i>Bp</i>137 were performed in comparison with other vaccine strains used in Latin America (<i>Bp&l...

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Autores principales: Bottero, Daniela, Gaillard, María Emilia, Basile, Laura A., Fritz, Mariana, Hozbor, Daniela Flavia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Exactas
Disease
Pertussis
Proteomic
Re-emergence
Vaccine strains
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84071
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84071
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Disease
Pertussis
Proteomic
Re-emergence
Vaccine strains
spellingShingle Ciencias Exactas
Disease
Pertussis
Proteomic
Re-emergence
Vaccine strains
Bottero, Daniela
Gaillard, María Emilia
Basile, Laura A.
Fritz, Mariana
Hozbor, Daniela Flavia
Genotypic and phenotypic characterization of Bordetella pertussis strains used in different vaccine formulations in Latin America
topic_facet Ciencias Exactas
Disease
Pertussis
Proteomic
Re-emergence
Vaccine strains
description Aim: To characterize <i>Bordetella pertussis</i> vaccine strains in comparison with current circulating bacteria. Methods and Results: Genomic and proteomic analyses of <i>Bp</i>137 were performed in comparison with other vaccine strains used in Latin America (<i>Bp</i>509 and <i>Bp</i>10536) and with the clinical Argentinean isolate <i>Bp</i>106. Tohama I strain was used as reference strain. Pulse-field gel electrophoresis (PFGE) and pertussis toxin promoter (<i>ptx</i>P) sequence analysis revealed that <i>Bp</i>137 groups with <i>Bp</i>509 in PFGE group III and contains <i>ptx</i>P2 sequence. Tohama I (group II) and <i>Bp</i>10536 (group I) contain <i>ptx</i>P1 sequence, while <i>Bp</i>106 belongs to a different PFGE cluster and contains <i>ptx</i>P3. Surface protein profiles diverged in at least 24 peptide subunits among the studied strains. From these 24 differential proteins, <i>Bp</i>10536 shared the expression of ten proteins with Tohama I and <i>Bp</i>509, but only three with <i>Bp</i>137. In contrast, seven proteins were detected exclusively in <i>Bp</i>137 and <i>Bp</i>106. Conclusions: <i>Bp</i>137 showed more features in common with the clinical isolate <i>Bp</i>106 than the other vaccine strains here included. Significance and Impact of the Study: The results presented show that the old strains included in vaccines are not all equal among them. These findings together with the data of circulating bacteria should be taken into account to select the best vaccine to be included in a national immunization programme.
format Articulo
Articulo
author Bottero, Daniela
Gaillard, María Emilia
Basile, Laura A.
Fritz, Mariana
Hozbor, Daniela Flavia
author_facet Bottero, Daniela
Gaillard, María Emilia
Basile, Laura A.
Fritz, Mariana
Hozbor, Daniela Flavia
author_sort Bottero, Daniela
title Genotypic and phenotypic characterization of Bordetella pertussis strains used in different vaccine formulations in Latin America
title_short Genotypic and phenotypic characterization of Bordetella pertussis strains used in different vaccine formulations in Latin America
title_full Genotypic and phenotypic characterization of Bordetella pertussis strains used in different vaccine formulations in Latin America
title_fullStr Genotypic and phenotypic characterization of Bordetella pertussis strains used in different vaccine formulations in Latin America
title_full_unstemmed Genotypic and phenotypic characterization of Bordetella pertussis strains used in different vaccine formulations in Latin America
title_sort genotypic and phenotypic characterization of bordetella pertussis strains used in different vaccine formulations in latin america
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/84071
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