Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality

Although <i>Bordetella pertussis</i> contains and transcribes loci encoding type III secretion system (TTSS) homologues, expression of TTSS-associated proteins has been reported only for non-laboratory-adapted Irish clinical isolates. Here we confirm such a result for clinical isolates o...

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Autores principales: Gaillard, María Emilia, Bottero, Daniela, Castuma, Cecilia, Basile, Laura A., Hozbor, Daniela Flavia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Ciencias Exactas
Bordetella pertussis
type III secretion system
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84045
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84045
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bordetella pertussis
type III secretion system
spellingShingle Ciencias Exactas
Bordetella pertussis
type III secretion system
Gaillard, María Emilia
Bottero, Daniela
Castuma, Cecilia
Basile, Laura A.
Hozbor, Daniela Flavia
Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality
topic_facet Ciencias Exactas
Bordetella pertussis
type III secretion system
description Although <i>Bordetella pertussis</i> contains and transcribes loci encoding type III secretion system (TTSS) homologues, expression of TTSS-associated proteins has been reported only for non-laboratory-adapted Irish clinical isolates. Here we confirm such a result for clinical isolates obtained from patients treated in Argentinean hospitals. Moreover, we demonstrate that the expression of TTSS-associated proteins is independent both of the year in which the isolate was obtained and of the types of polymorphic alleles for other virulence factors but is dependent on environmental growth conditions. Interestingly, we observed that TTSS-associated protein expression is lost after successive <i>in vitro</i> passages but becomes operative again when bacteria come into contact with the host. This <i>in vivo</i> activation of TTSS expression was observed not only for clinical isolates previously adapted to the laboratory after successive <i>in vitro</i> passages but also for vaccine strains that did not express the system <i>in vitro</i>. The reversibility of TTSS expression, demonstrated by its switching off-on when the bacterium comes into contact with the host, appears to be an adaptive response of this pathogen.
format Articulo
Articulo
author Gaillard, María Emilia
Bottero, Daniela
Castuma, Cecilia
Basile, Laura A.
Hozbor, Daniela Flavia
author_facet Gaillard, María Emilia
Bottero, Daniela
Castuma, Cecilia
Basile, Laura A.
Hozbor, Daniela Flavia
author_sort Gaillard, María Emilia
title Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality
title_short Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality
title_full Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality
title_fullStr Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality
title_full_unstemmed Laboratory adaptation of Bordetella pertussis is associated with the loss of type three secretion system functionality
title_sort laboratory adaptation of bordetella pertussis is associated with the loss of type three secretion system functionality
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/84045
work_keys_str_mv AT gaillardmariaemilia laboratoryadaptationofbordetellapertussisisassociatedwiththelossoftypethreesecretionsystemfunctionality
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