The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine

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Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strat...

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Autores principales: Muglia, Cecilia Isabel, Mercer, Natalia, Toscano, M. A., Schattner, M., Pozner, Raúl Ernesto, Cerliani, J. P., Papa Gobbi, Rodrigo, Rabinovich, G. A., Docena, Guillermo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Ciencias Exactas
Apoptosis
Enterocytes
Galectin-1
Mucosa
Small bowel
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83984
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83984
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Apoptosis
Enterocytes
Galectin-1
Mucosa
Small bowel
spellingShingle Ciencias Exactas
Apoptosis
Enterocytes
Galectin-1
Mucosa
Small bowel
Muglia, Cecilia Isabel
Mercer, Natalia
Toscano, M. A.
Schattner, M.
Pozner, Raúl Ernesto
Cerliani, J. P.
Papa Gobbi, Rodrigo
Rabinovich, G. A.
Docena, Guillermo
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
topic_facet Ciencias Exactas
Apoptosis
Enterocytes
Galectin-1
Mucosa
Small bowel
description Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function.
format Articulo
Articulo
author Muglia, Cecilia Isabel
Mercer, Natalia
Toscano, M. A.
Schattner, M.
Pozner, Raúl Ernesto
Cerliani, J. P.
Papa Gobbi, Rodrigo
Rabinovich, G. A.
Docena, Guillermo
author_facet Muglia, Cecilia Isabel
Mercer, Natalia
Toscano, M. A.
Schattner, M.
Pozner, Raúl Ernesto
Cerliani, J. P.
Papa Gobbi, Rodrigo
Rabinovich, G. A.
Docena, Guillermo
author_sort Muglia, Cecilia Isabel
title The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title_short The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title_full The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title_fullStr The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title_full_unstemmed The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title_sort glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/83984
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