The nodulation of alfalfa by the acid-tolerant Rhizobium sp. strain LPU83 does not require sulfated forms of lipochitooligosaccharide nodulation signals

The induction of root nodules by the majority of rhizobia has a strict requirement for the secretion of symbiosis-specific lipochitooligosaccharides (nodulation factors [NFs]). The nature of the chemical substitution on the NFs depends on the particular rhizobium and contributes to the host specific...

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Autores principales: Torres Tejerizo, Gonzalo Arturo, Del Papa, María Florencia, Eugenia Soria-Diaz, M., Draghi, Walter Omar, Lozano, Mauricio Javier, Giusti, María de los Ángeles, Manyani, Hamid, Megías, Manuel, Serrano, Antonio Gil, Pühler, Alfred, Niehaus, Karsten, Lagares, Antonio, Pistorio, Mariano
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Ciencias Astronómicas
alfalfa nodulation
sulfate decoration
nodulation factors
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83958
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83958
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Astronómicas
alfalfa nodulation
sulfate decoration
nodulation factors
spellingShingle Ciencias Astronómicas
alfalfa nodulation
sulfate decoration
nodulation factors
Torres Tejerizo, Gonzalo Arturo
Del Papa, María Florencia
Eugenia Soria-Diaz, M.
Draghi, Walter Omar
Lozano, Mauricio Javier
Giusti, María de los Ángeles
Manyani, Hamid
Megías, Manuel
Serrano, Antonio Gil
Pühler, Alfred
Niehaus, Karsten
Lagares, Antonio
Pistorio, Mariano
The nodulation of alfalfa by the acid-tolerant Rhizobium sp. strain LPU83 does not require sulfated forms of lipochitooligosaccharide nodulation signals
topic_facet Ciencias Astronómicas
alfalfa nodulation
sulfate decoration
nodulation factors
description The induction of root nodules by the majority of rhizobia has a strict requirement for the secretion of symbiosis-specific lipochitooligosaccharides (nodulation factors [NFs]). The nature of the chemical substitution on the NFs depends on the particular rhizobium and contributes to the host specificity imparted by the NFs. We present here a description of the genetic organization of the nod gene cluster and the characterization of the chemical structure of the NFs associated with the broad-host-range Rhizobium sp. strain LPU83, a bacterium capable of nodulating at least alfalfa, bean, and Leucena leucocephala. The nod gene cluster was located on the plasmid pLPU83b. The organization of the cluster showed synteny with those of the alfalfanodulating rhizobia, Sinorhizobium meliloti and Sinorhizobium medicae. Interestingly, the strongest sequence similarity observed was between the partial nod sequences of Rhizobium mongolense USDA 1844 and the corresponding LPU83 nod genes sequences. The phylogenetic analysis of the intergenic region nodEG positions strain LPU83 and the type strain R. mongolense 1844 in the same branch, which indicates that Rhizobium sp. strain LPU83 might represent an early alfalfa-nodulating genotype. The NF chemical structures obtained for the wild-type strain consist of a trimeric, tetrameric, and pentameric chitin backbone that shares some substitutions with both alfalfa- and bean-nodulating rhizobia. Remarkably, while in strain LPU83 most of the NFs were sulfated in their reducing terminal residue, none of the NFs isolated from the nodH mutant LPU83-H were sulfated. The evidence obtained supports the notion that the sulfate decoration of NFs in LPU83 is not necessary for alfalfa nodulation.
format Articulo
Articulo
author Torres Tejerizo, Gonzalo Arturo
Del Papa, María Florencia
Eugenia Soria-Diaz, M.
Draghi, Walter Omar
Lozano, Mauricio Javier
Giusti, María de los Ángeles
Manyani, Hamid
Megías, Manuel
Serrano, Antonio Gil
Pühler, Alfred
Niehaus, Karsten
Lagares, Antonio
Pistorio, Mariano
author_facet Torres Tejerizo, Gonzalo Arturo
Del Papa, María Florencia
Eugenia Soria-Diaz, M.
Draghi, Walter Omar
Lozano, Mauricio Javier
Giusti, María de los Ángeles
Manyani, Hamid
Megías, Manuel
Serrano, Antonio Gil
Pühler, Alfred
Niehaus, Karsten
Lagares, Antonio
Pistorio, Mariano
author_sort Torres Tejerizo, Gonzalo Arturo
title The nodulation of alfalfa by the acid-tolerant Rhizobium sp. strain LPU83 does not require sulfated forms of lipochitooligosaccharide nodulation signals
title_short The nodulation of alfalfa by the acid-tolerant Rhizobium sp. strain LPU83 does not require sulfated forms of lipochitooligosaccharide nodulation signals
title_full The nodulation of alfalfa by the acid-tolerant Rhizobium sp. strain LPU83 does not require sulfated forms of lipochitooligosaccharide nodulation signals
title_fullStr The nodulation of alfalfa by the acid-tolerant Rhizobium sp. strain LPU83 does not require sulfated forms of lipochitooligosaccharide nodulation signals
title_full_unstemmed The nodulation of alfalfa by the acid-tolerant Rhizobium sp. strain LPU83 does not require sulfated forms of lipochitooligosaccharide nodulation signals
title_sort nodulation of alfalfa by the acid-tolerant rhizobium sp. strain lpu83 does not require sulfated forms of lipochitooligosaccharide nodulation signals
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/83958
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