Triptolide modulates adriamycin sensitivity via regulating Mir-21 and Bcl-2 expression in K562/A02 cell line
Drug resistance is a major obstacle for successful treatment of leukemia. Increasing evidence suggests that microRNA-21 (miR-21) is over-expressed in K562/A02 cell line, promoting drug resistance. The aim of our present study is to investigate the reversal effects of triptolide on drug resistance in...
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| Autores principales: | , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8395 http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_7.pdf |
| Aporte de: |
| Sumario: | Drug resistance is a major obstacle for successful treatment of leukemia. Increasing evidence suggests that microRNA-21 (miR-21) is over-expressed in K562/A02 cell line, promoting drug resistance. The aim of our present study is to investigate the reversal effects of triptolide on drug resistance in adriamycin-resistant cells. Cell viability was measured by MTT assays and adriamycin induced apoptosis was evaluated by flow cytometry. Levels of miR-21 quantified by real-time PCR. Bcl-2 protein level were measured by western blot. TPL enhanced sensitivity of K562/A02 cells to adriamycin and promoted adriamycin–induced apoptosis. Levels of miR-21 and Bcl-2 was significantly decreased after triptolide treatment. Transfection with anti-miR-21, a significant up-regulation of sensitivity to adriamycin and a significant down-regulation of Bcl-2 protein level was noted in K562/A02 cells. Our study suggests that triptolide significantly sensitizes K562/A02 cell to adriamycin by inducing apoptosis and these effects of triptolide may be due to its down-regulation of miR-21. |
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