Determination of meropenem in rabbit plasma by LC-MS/MS
A sensitive and selective liquid chromatography tandem mass spectrometry (LC–MS/MS) method for determination of meropenem in rabbit plasma was developed. After addition of triazolam as internal standard (IS), protein precipitation by acetonitrile was used in sample preparation. Chromatographic separ...
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| Autores principales: | , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8392 http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_4.pdf |
| Aporte de: |
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I19-R120-10915-8392 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia Farmacocinética antibiótico Plasma Cromatografía Liquida Espectrometría de Masas |
| spellingShingle |
Farmacia Farmacocinética antibiótico Plasma Cromatografía Liquida Espectrometría de Masas Lin, Guanyang Zhang, Haiyang Xue, Feng Li, Wenjuan Liu, Naihua Chen, Lianguo Wang, Xianqin Xu, Ren-ai Ma, Jianshe Determination of meropenem in rabbit plasma by LC-MS/MS |
| topic_facet |
Farmacia Farmacocinética antibiótico Plasma Cromatografía Liquida Espectrometría de Masas |
| description |
A sensitive and selective liquid chromatography tandem mass spectrometry (LC–MS/MS) method for determination of meropenem in rabbit plasma was developed. After addition of triazolam as internal standard (IS), protein precipitation by acetonitrile was used in sample preparation. Chromatographic separation was achieved on a Restek Allure (TM) PFP Propyl (2.1 mm × 100 mm, 5 μm) column with acetonitrile-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used to quantification using target fragment ions m/z 384.1 → 339.9 for meropenem and m/z 342.7 → 307.8 for the IS. Calibration plots were linear over the range of 0.1-40 μg/mL for meropenem in plasma. Lower limit of quantification (LLOQ) for meropenem was 0.1 μg/mL. Mean recovery of meropenem from plasma was in the range 85.6 %-96.5 %. CV of intra-day and inter-day precision were both less than 15 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of meropenem in rabbit plasma. |
| format |
Articulo Articulo |
| author |
Lin, Guanyang Zhang, Haiyang Xue, Feng Li, Wenjuan Liu, Naihua Chen, Lianguo Wang, Xianqin Xu, Ren-ai Ma, Jianshe |
| author_facet |
Lin, Guanyang Zhang, Haiyang Xue, Feng Li, Wenjuan Liu, Naihua Chen, Lianguo Wang, Xianqin Xu, Ren-ai Ma, Jianshe |
| author_sort |
Lin, Guanyang |
| title |
Determination of meropenem in rabbit plasma by LC-MS/MS |
| title_short |
Determination of meropenem in rabbit plasma by LC-MS/MS |
| title_full |
Determination of meropenem in rabbit plasma by LC-MS/MS |
| title_fullStr |
Determination of meropenem in rabbit plasma by LC-MS/MS |
| title_full_unstemmed |
Determination of meropenem in rabbit plasma by LC-MS/MS |
| title_sort |
determination of meropenem in rabbit plasma by lc-ms/ms |
| publishDate |
2011 |
| url |
http://sedici.unlp.edu.ar/handle/10915/8392 http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_4.pdf |
| work_keys_str_mv |
AT linguanyang determinationofmeropeneminrabbitplasmabylcmsms AT zhanghaiyang determinationofmeropeneminrabbitplasmabylcmsms AT xuefeng determinationofmeropeneminrabbitplasmabylcmsms AT liwenjuan determinationofmeropeneminrabbitplasmabylcmsms AT liunaihua determinationofmeropeneminrabbitplasmabylcmsms AT chenlianguo determinationofmeropeneminrabbitplasmabylcmsms AT wangxianqin determinationofmeropeneminrabbitplasmabylcmsms AT xurenai determinationofmeropeneminrabbitplasmabylcmsms AT majianshe determinationofmeropeneminrabbitplasmabylcmsms |
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Repositorios |
| _version_ |
1764820488187543553 |