Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean

Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in com...

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Detalles Bibliográficos
Autores principales: Althabegoiti, María Julia, Covelli, Julieta Mariana, Pérez Giménez, Julieta, Quelas, Juan Ignacio, Mongiardini, Elías Javier, López, María Florencia, López García, Silvina Laura, Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Biología
Flagellin
Soil
Swimming
Symbiosis
Water content
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83919
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83919
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Flagellin
Soil
Swimming
Symbiosis
Water content
spellingShingle Biología
Flagellin
Soil
Swimming
Symbiosis
Water content
Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Quelas, Juan Ignacio
Mongiardini, Elías Javier
López, María Florencia
López García, Silvina Laura
Lodeiro, Aníbal Roberto
Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
topic_facet Biología
Flagellin
Soil
Swimming
Symbiosis
Water content
description Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodulation, we obtained mutants deleted in fliCI-II and/or fliC1-4 in the genomic backgrounds of two derivatives from the reference strain USDA 110: the streptomycin-resistant derivative LP 3004 and its more motile derivative LP 3008. All mutations diminished swimming motility. When each mutant was co-inoculated with the parental strain on soybean plants cultivated in vermiculite either at field capacity or flooded, their competitiveness differed according to the flagellin altered. ΔfliCI-II mutants were more competitive, occupying 64-80% of the nodules, while ΔfliC1-4 mutants occupied 45-49% of the nodules. Occupation by the nonmotile double mutant decreased from 55% to 11% as the water content of the vermiculite increased from 85% to 95% field capacity to flooding. These results indicate that the influence of motility on competitiveness depended on the water status of the rooting substrate.
format Articulo
Articulo
author Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Quelas, Juan Ignacio
Mongiardini, Elías Javier
López, María Florencia
López García, Silvina Laura
Lodeiro, Aníbal Roberto
author_facet Althabegoiti, María Julia
Covelli, Julieta Mariana
Pérez Giménez, Julieta
Quelas, Juan Ignacio
Mongiardini, Elías Javier
López, María Florencia
López García, Silvina Laura
Lodeiro, Aníbal Roberto
author_sort Althabegoiti, María Julia
title Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title_short Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title_full Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title_fullStr Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title_full_unstemmed Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean
title_sort analysis of the role of the two flagella of bradyrhizobium japonicum in competition for nodulation of soybean
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/83919
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