Chemical composition of n-BuOH extract of Potentilla anserina L. and its protective effect of EAhy926 endothelial cells under hypoxia

The protective role of n-BuOH extract of Potentilla anserina roots was measured by MTT method and colorimetric method on human umbilical vein endothelial cells (EAhy926) under hypoxia injury. The extract tested (3 mg/mL, 1.5 mg/mL) remarkably increased cell viability, the activity of superoxide dism...

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Detalles Bibliográficos
Autores principales: Liu, Dailin, Wang, Shu, Zhang, Ling, Li, Lingzhi
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Isoflavonas
Potentilla anserina
Células Endoteliales
Venas Umbilicales
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8391
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_3.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8391
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Isoflavonas
Potentilla anserina
Células Endoteliales
Venas Umbilicales
spellingShingle Farmacia
Isoflavonas
Potentilla anserina
Células Endoteliales
Venas Umbilicales
Liu, Dailin
Wang, Shu
Zhang, Ling
Li, Lingzhi
Chemical composition of n-BuOH extract of Potentilla anserina L. and its protective effect of EAhy926 endothelial cells under hypoxia
topic_facet Farmacia
Isoflavonas
Potentilla anserina
Células Endoteliales
Venas Umbilicales
description The protective role of n-BuOH extract of Potentilla anserina roots was measured by MTT method and colorimetric method on human umbilical vein endothelial cells (EAhy926) under hypoxia injury. The extract tested (3 mg/mL, 1.5 mg/mL) remarkably increased cell viability, the activity of superoxide dismutase (SOD) and the concentration of nitrogen monoxidum (NO), and at same time reduced the release of lactate dehydrogenase (LDH) and endothelin (ET-1) in cells during hypoxia injury. From this extraction, five compounds were isolated and determined as adenosine (1), daidzin (2), puerarin (3), 3'- methoxypuerarin (4) and daidzein 8-C-apiosyl glucoside (5) on the basis of physico-chemical properties and spectroscopic analysis, including 1D- and 2D-NMR spectral data. Compound 2-5 were isolated from genus Potentilla for the first time. Compound 1 was first isolated from the title plant.
format Articulo
Articulo
author Liu, Dailin
Wang, Shu
Zhang, Ling
Li, Lingzhi
author_facet Liu, Dailin
Wang, Shu
Zhang, Ling
Li, Lingzhi
author_sort Liu, Dailin
title Chemical composition of n-BuOH extract of Potentilla anserina L. and its protective effect of EAhy926 endothelial cells under hypoxia
title_short Chemical composition of n-BuOH extract of Potentilla anserina L. and its protective effect of EAhy926 endothelial cells under hypoxia
title_full Chemical composition of n-BuOH extract of Potentilla anserina L. and its protective effect of EAhy926 endothelial cells under hypoxia
title_fullStr Chemical composition of n-BuOH extract of Potentilla anserina L. and its protective effect of EAhy926 endothelial cells under hypoxia
title_full_unstemmed Chemical composition of n-BuOH extract of Potentilla anserina L. and its protective effect of EAhy926 endothelial cells under hypoxia
title_sort chemical composition of n-buoh extract of potentilla anserina l. and its protective effect of eahy926 endothelial cells under hypoxia
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8391
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_3.pdf
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AT wangshu chemicalcompositionofnbuohextractofpotentillaanserinalanditsprotectiveeffectofeahy926endothelialcellsunderhypoxia
AT zhangling chemicalcompositionofnbuohextractofpotentillaanserinalanditsprotectiveeffectofeahy926endothelialcellsunderhypoxia
AT lilingzhi chemicalcompositionofnbuohextractofpotentillaanserinalanditsprotectiveeffectofeahy926endothelialcellsunderhypoxia
bdutipo_str Repositorios
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