Determination of meloxicam in human plasma administrated with four drugs by LC method: application to a pilot bioavailability study

The development and validation of a simple and accurate method by HPLC-UV to quantify meloxicam (MLX) in human plasma and its application to comparative bioavailability study between MLX formulation and manipulated meloxicam + prednisone + cyclobenzaprine + diacerein + hidroxychloroquine is describe...

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Detalles Bibliográficos
Autores principales:	Leal, Leila B., Bedor, Danilo C. G., Melo, Elayne K. S., Oliveira, Eduardo J.A., Santana, Davi P.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia Farmacocinética Cromatografía de Fase Inversa Agentes Antiinflamatorios no Esteroides Plasma
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8390 http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_2.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

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institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia Farmacocinética Cromatografía de Fase Inversa Agentes Antiinflamatorios no Esteroides Plasma
spellingShingle	Farmacia Farmacocinética Cromatografía de Fase Inversa Agentes Antiinflamatorios no Esteroides Plasma Leal, Leila B. Bedor, Danilo C. G. Melo, Elayne K. S. Oliveira, Eduardo J.A. Santana, Davi P. Determination of meloxicam in human plasma administrated with four drugs by LC method: application to a pilot bioavailability study
topic_facet	Farmacia Farmacocinética Cromatografía de Fase Inversa Agentes Antiinflamatorios no Esteroides Plasma
description	The development and validation of a simple and accurate method by HPLC-UV to quantify meloxicam (MLX) in human plasma and its application to comparative bioavailability study between MLX formulation and manipulated meloxicam + prednisone + cyclobenzaprine + diacerein + hidroxychloroquine is described. MLX and the internal standard (piroxicam) were extracted from plasma using protein precipitation. Chromatographic separation of meloxicam, piroxicam, other active ingredients, diacerein metabolite (Rhein) and plasma interferents was achieved with a C18 column, using a mobile phase of 20 mM sodium Hydrogen pH 3.0 and acetonitrile, with detection at 360 nm and retention times of 4.7, 3.7 and 4.1 min, respectively. The method was linear over the concentration range of 50 to 3000 ng/mL, meloxicam and piroxicam had an average recovery from plasma of 96 and 97 %, respectively. The precision and accuracy (intra-, inter-day) were less than 6 %. The method was successfully applied to a pilot pharmacokinetic study.
format	Articulo Articulo
author	Leal, Leila B. Bedor, Danilo C. G. Melo, Elayne K. S. Oliveira, Eduardo J.A. Santana, Davi P.
author_facet	Leal, Leila B. Bedor, Danilo C. G. Melo, Elayne K. S. Oliveira, Eduardo J.A. Santana, Davi P.
author_sort	Leal, Leila B.
title	Determination of meloxicam in human plasma administrated with four drugs by LC method: application to a pilot bioavailability study
title_short	Determination of meloxicam in human plasma administrated with four drugs by LC method: application to a pilot bioavailability study
title_full	Determination of meloxicam in human plasma administrated with four drugs by LC method: application to a pilot bioavailability study
title_fullStr	Determination of meloxicam in human plasma administrated with four drugs by LC method: application to a pilot bioavailability study
title_full_unstemmed	Determination of meloxicam in human plasma administrated with four drugs by LC method: application to a pilot bioavailability study
title_sort	determination of meloxicam in human plasma administrated with four drugs by lc method: application to a pilot bioavailability study
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8390 http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_2.pdf
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bdutipo_str	Repositorios
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Determination of meloxicam in human plasma administrated with four drugs by LC method: application to a pilot bioavailability study

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