Determination of ketamine in rabbit plasma by gradient elution liquid chromatography/electrospray mass spectrometry

A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of ketamine in rabbit plasma using one-step protein precipitation was developed and validated. After addition of methadone as internal standard (IS), protein precipitation by acetonitrile...

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Autores principales: Hu, Lufeng, Wang, Xianqin, Wang, Xuebao, Zeng, Ming, Ma, Jianshe, Li, Wei
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
conejo
Espectrometría de Masas
Cromatografía
Plasma
Ketamina
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8384
http://www.latamjpharm.org/resumenes/30/9/LAJOP_30_9_2_1.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8384
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
conejo
Espectrometría de Masas
Cromatografía
Plasma
Ketamina
spellingShingle Farmacia
conejo
Espectrometría de Masas
Cromatografía
Plasma
Ketamina
Hu, Lufeng
Wang, Xianqin
Wang, Xuebao
Zeng, Ming
Ma, Jianshe
Li, Wei
Determination of ketamine in rabbit plasma by gradient elution liquid chromatography/electrospray mass spectrometry
topic_facet Farmacia
conejo
Espectrometría de Masas
Cromatografía
Plasma
Ketamina
description A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of ketamine in rabbit plasma using one-step protein precipitation was developed and validated. After addition of methadone as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographically separation was achieved on an SB-C18 (2.1 mm × 50 mm, 3.5 μm) column with methanol-0.1 % formic acid as the mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used to quantification using target fragment ions m/z 237.7 → 219.7 for ketamine and m/z 309.9 → 264.8 for the IS. Calibration plots were linear over the range of 5-1000 ng/mL for ketamine in rabbit plasma. Lower limit of quantification (LLOQ) for ketamine was 5 ng/mL. Mean recovery of ketamine from plasma was in the range of 97.5-100.1 %. RSD of intra-day and inter-day precision were both less than 11 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of ketamine in rabbit plasma.
format Articulo
Comunicacion
author Hu, Lufeng
Wang, Xianqin
Wang, Xuebao
Zeng, Ming
Ma, Jianshe
Li, Wei
author_facet Hu, Lufeng
Wang, Xianqin
Wang, Xuebao
Zeng, Ming
Ma, Jianshe
Li, Wei
author_sort Hu, Lufeng
title Determination of ketamine in rabbit plasma by gradient elution liquid chromatography/electrospray mass spectrometry
title_short Determination of ketamine in rabbit plasma by gradient elution liquid chromatography/electrospray mass spectrometry
title_full Determination of ketamine in rabbit plasma by gradient elution liquid chromatography/electrospray mass spectrometry
title_fullStr Determination of ketamine in rabbit plasma by gradient elution liquid chromatography/electrospray mass spectrometry
title_full_unstemmed Determination of ketamine in rabbit plasma by gradient elution liquid chromatography/electrospray mass spectrometry
title_sort determination of ketamine in rabbit plasma by gradient elution liquid chromatography/electrospray mass spectrometry
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8384
http://www.latamjpharm.org/resumenes/30/9/LAJOP_30_9_2_1.pdf
work_keys_str_mv AT hulufeng determinationofketamineinrabbitplasmabygradientelutionliquidchromatographyelectrospraymassspectrometry
AT wangxianqin determinationofketamineinrabbitplasmabygradientelutionliquidchromatographyelectrospraymassspectrometry
AT wangxuebao determinationofketamineinrabbitplasmabygradientelutionliquidchromatographyelectrospraymassspectrometry
AT zengming determinationofketamineinrabbitplasmabygradientelutionliquidchromatographyelectrospraymassspectrometry
AT majianshe determinationofketamineinrabbitplasmabygradientelutionliquidchromatographyelectrospraymassspectrometry
AT liwei determinationofketamineinrabbitplasmabygradientelutionliquidchromatographyelectrospraymassspectrometry
bdutipo_str Repositorios
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