Determination of sulpiride in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study

A sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method for determination of sulpiride in rabbit plasma was developed and validated. The analyte and internal standard (IS) were extracted from plasma by liquid-liquid extraction using ethyl acetate, and chromatography involved...

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Autores principales: Lin, Guanyang, Wu, Haiya, Wang, Zhibin, Zhang, Haiyang, Chien, Lianguo, Wang, Xianqin, Hu Lufeng, Ma, Jianshe
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Sulpirida
Farmacocinética
Conejos
Plasma
Cromatografía Liquida
Espectrometría de Masas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8373
http://www.latamjpharm.org/resumenes/30/9/LAJOP_30_9_1_17.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8373
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Sulpirida
Farmacocinética
Conejos
Plasma
Cromatografía Liquida
Espectrometría de Masas
spellingShingle Farmacia
Sulpirida
Farmacocinética
Conejos
Plasma
Cromatografía Liquida
Espectrometría de Masas
Lin, Guanyang
Wu, Haiya
Wang, Zhibin
Zhang, Haiyang
Chien, Lianguo
Wang, Xianqin
Hu Lufeng
Ma, Jianshe
Determination of sulpiride in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study
topic_facet Farmacia
Sulpirida
Farmacocinética
Conejos
Plasma
Cromatografía Liquida
Espectrometría de Masas
description A sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method for determination of sulpiride in rabbit plasma was developed and validated. The analyte and internal standard (IS) were extracted from plasma by liquid-liquid extraction using ethyl acetate, and chromatography involved Agilent Extend-C18 column (2.1 mm x 50 mm, 3.5 μm) using 0.2 % formic acid in water and acetonitrile (60: 40, v/v) as a mobile phase. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragmentions m/z 342.0 for sulpiride and m/z 294.8 for estazolam (internal standard, IS). The assay was linear over the range of 10-2000 ng/mL for sulpiride, with a lower limit of quantitation (LLOQ) of 10 ng/mL for sulpiride. Intraand inter-day precisions were less than 12 % and the accuracies were in the range of 94.1-108.7 % for sulpiride. This developed method was successfully applied for the determination of sulpiride in rabbit plasma for pharmacokinetic study.
format Articulo
Articulo
author Lin, Guanyang
Wu, Haiya
Wang, Zhibin
Zhang, Haiyang
Chien, Lianguo
Wang, Xianqin
Hu Lufeng
Ma, Jianshe
author_facet Lin, Guanyang
Wu, Haiya
Wang, Zhibin
Zhang, Haiyang
Chien, Lianguo
Wang, Xianqin
Hu Lufeng
Ma, Jianshe
author_sort Lin, Guanyang
title Determination of sulpiride in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study
title_short Determination of sulpiride in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study
title_full Determination of sulpiride in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study
title_fullStr Determination of sulpiride in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study
title_full_unstemmed Determination of sulpiride in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study
title_sort determination of sulpiride in rabbit plasma by lc-esi-ms and its application to a pharmacokinetic study
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8373
http://www.latamjpharm.org/resumenes/30/9/LAJOP_30_9_1_17.pdf
work_keys_str_mv AT linguanyang determinationofsulpirideinrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy
AT wuhaiya determinationofsulpirideinrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy
AT wangzhibin determinationofsulpirideinrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy
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AT hulufeng determinationofsulpirideinrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy
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bdutipo_str Repositorios
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