Characterization of ichthyocidal activity of Pfiesteria piscicida: Dependence on the dinospore cell density

The ichthyocidal activity of <i>Pfiesteria piscicida</i> dinospores was examined in an aquarium bioassay format by exposing fish to either <i>Pfiesteria</i>-containing environmental sediments or clonal <i>P. piscicida</i>. The presence of <i>Pfiesteria</i...

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Autores principales: Drgon, Tomás, Saito, Keiko, Gillevet, Patrick M., Sikaroodi, Masoumeh, Whitaker, Brent, Krupatkina, Danara N., Argemi, Federico, Vasta, Gerardo R.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Ciencias Naturales
Pfiesteria piscicida
ichthyocidal activity
dinospores
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83587
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83587
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Pfiesteria piscicida
ichthyocidal activity
dinospores
spellingShingle Ciencias Naturales
Pfiesteria piscicida
ichthyocidal activity
dinospores
Drgon, Tomás
Saito, Keiko
Gillevet, Patrick M.
Sikaroodi, Masoumeh
Whitaker, Brent
Krupatkina, Danara N.
Argemi, Federico
Vasta, Gerardo R.
Characterization of ichthyocidal activity of Pfiesteria piscicida: Dependence on the dinospore cell density
topic_facet Ciencias Naturales
Pfiesteria piscicida
ichthyocidal activity
dinospores
description The ichthyocidal activity of <i>Pfiesteria piscicida</i> dinospores was examined in an aquarium bioassay format by exposing fish to either <i>Pfiesteria</i>-containing environmental sediments or clonal <i>P. piscicida</i>. The presence of <i>Pfiesteria</i> spp. and the complexity of the microbial assemblage in the bioassay were assessed by molecular approaches. Cell-free water from bioassays that yielded significant fish mortality failed to show ichthyocidal activity. Histopathological examination of moribund and dead fish failed to reveal the skin lesions reported elsewhere. Fish larvae within "cages" of variable mesh sizes were killed in those where the pore size exceeded that of <i>Pfiesteria</i> dinospores. In vitro exposure of fish larvae to clonal <i>P. piscicida</i> indicated that fish mortality was directly proportional to the dinospore cell density. Dinospores clustered around the mouth, eyes, and operculi, suggesting that fish health may be affected by their direct interaction with skin, gill epithelia, or mucous surfaces. Molecular fingerprinting revealed the presence of a very diverse microbial community of bacteria, protists, and fungi within bioassay aquaria containing environmental sediments. Some components of the microbial community were identified as potential fish pathogens, preventing the rigorous identification of <i>Pfiesteria</i> spp. as the only cause of fish death. In summary, our results strongly suggest (i) that this aquarium bioassay format, which has been extensively reported in the literature, is unsuitable to accurately assess the ichthyocidal activity of <i>Pfiesteria</i> spp. and (ii) that the ichthyocidal activity of <i>Pfiesteria</i> spp. is mostly due to direct interactions of the zoospores with fish skin and gill epithelia rather than to soluble factors.
format Articulo
Articulo
author Drgon, Tomás
Saito, Keiko
Gillevet, Patrick M.
Sikaroodi, Masoumeh
Whitaker, Brent
Krupatkina, Danara N.
Argemi, Federico
Vasta, Gerardo R.
author_facet Drgon, Tomás
Saito, Keiko
Gillevet, Patrick M.
Sikaroodi, Masoumeh
Whitaker, Brent
Krupatkina, Danara N.
Argemi, Federico
Vasta, Gerardo R.
author_sort Drgon, Tomás
title Characterization of ichthyocidal activity of Pfiesteria piscicida: Dependence on the dinospore cell density
title_short Characterization of ichthyocidal activity of Pfiesteria piscicida: Dependence on the dinospore cell density
title_full Characterization of ichthyocidal activity of Pfiesteria piscicida: Dependence on the dinospore cell density
title_fullStr Characterization of ichthyocidal activity of Pfiesteria piscicida: Dependence on the dinospore cell density
title_full_unstemmed Characterization of ichthyocidal activity of Pfiesteria piscicida: Dependence on the dinospore cell density
title_sort characterization of ichthyocidal activity of pfiesteria piscicida: dependence on the dinospore cell density
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/83587
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