Rhizobium tropici response to acidity involves activation of glutathione synthesis

Rhizobium tropici CIAT899 displays intrinsic tolerance to acidity, and efficiently nodulates Phaseolus vulgaris at low pH. By characterizing a gshB mutant strain, glutathione has been previously demonstrated to be essential for R. tropici tolerance to acid stress. The wild-type gshB gene region has...

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Autores principales: Muglia, Cecilia Isabel, Grasso, Daniel Horacio, Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83540
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id I19-R120-10915-83540
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Rhizobium tropici
spellingShingle Biología
Rhizobium tropici
Muglia, Cecilia Isabel
Grasso, Daniel Horacio
Aguilar, Orlando Mario
Rhizobium tropici response to acidity involves activation of glutathione synthesis
topic_facet Biología
Rhizobium tropici
description Rhizobium tropici CIAT899 displays intrinsic tolerance to acidity, and efficiently nodulates Phaseolus vulgaris at low pH. By characterizing a gshB mutant strain, glutathione has been previously demonstrated to be essential for R. tropici tolerance to acid stress. The wild-type gshB gene region has been cloned and its transcription profile has been characterized by using quantitative real-time PCR and transcriptional gene fusions. Activation of the gshB gene under acid-stress conditions was demonstrated. gshB is also induced by UV irradiation. Upstream from gshB a putative σ 70 promoter element and an inverted repeat sequence were identified, which are proposed to be involved in expression under neutral and acidic conditions, respectively. Gel retardation assays indicate that transcription in acid conditions may involve protein binding to an upstream regulatory region.
format Articulo
Articulo
author Muglia, Cecilia Isabel
Grasso, Daniel Horacio
Aguilar, Orlando Mario
author_facet Muglia, Cecilia Isabel
Grasso, Daniel Horacio
Aguilar, Orlando Mario
author_sort Muglia, Cecilia Isabel
title Rhizobium tropici response to acidity involves activation of glutathione synthesis
title_short Rhizobium tropici response to acidity involves activation of glutathione synthesis
title_full Rhizobium tropici response to acidity involves activation of glutathione synthesis
title_fullStr Rhizobium tropici response to acidity involves activation of glutathione synthesis
title_full_unstemmed Rhizobium tropici response to acidity involves activation of glutathione synthesis
title_sort rhizobium tropici response to acidity involves activation of glutathione synthesis
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/83540
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AT grassodanielhoracio rhizobiumtropiciresponsetoacidityinvolvesactivationofglutathionesynthesis
AT aguilarorlandomario rhizobiumtropiciresponsetoacidityinvolvesactivationofglutathionesynthesis
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