Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor

The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR m...

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Autores principales: Berra Romani, Roberto, Raqeeb, Abdul, Laforenza, Umberto, Scaffino, Manuela Federica, Moccia, Francesco, Avelino-Cruz, Josè Everardo, Oldani, Amanda, Coltrini, Daniela, Milesi, Verónica, Taglietti, Vanni, Tanzi, Franco
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Química
Ca2+-sensing receptor
Cardiac function
Cardiac microvascular endothelial cells
Extracellular Ca2+
Fura-2/AM
Inositol 1,4,5-trisphosphate
Na+/Ca2+ exchanger
Phospholipase C
Vascular tone
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83531
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83531
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Ca2+-sensing receptor
Cardiac function
Cardiac microvascular endothelial cells
Extracellular Ca2+
Fura-2/AM
Inositol 1,4,5-trisphosphate
Na+/Ca2+ exchanger
Phospholipase C
Vascular tone
spellingShingle Química
Ca2+-sensing receptor
Cardiac function
Cardiac microvascular endothelial cells
Extracellular Ca2+
Fura-2/AM
Inositol 1,4,5-trisphosphate
Na+/Ca2+ exchanger
Phospholipase C
Vascular tone
Berra Romani, Roberto
Raqeeb, Abdul
Laforenza, Umberto
Scaffino, Manuela Federica
Moccia, Francesco
Avelino-Cruz, Josè Everardo
Oldani, Amanda
Coltrini, Daniela
Milesi, Verónica
Taglietti, Vanni
Tanzi, Franco
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
topic_facet Química
Ca2+-sensing receptor
Cardiac function
Cardiac microvascular endothelial cells
Extracellular Ca2+
Fura-2/AM
Inositol 1,4,5-trisphosphate
Na+/Ca2+ exchanger
Phospholipase C
Vascular tone
description The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores.
format Articulo
Articulo
author Berra Romani, Roberto
Raqeeb, Abdul
Laforenza, Umberto
Scaffino, Manuela Federica
Moccia, Francesco
Avelino-Cruz, Josè Everardo
Oldani, Amanda
Coltrini, Daniela
Milesi, Verónica
Taglietti, Vanni
Tanzi, Franco
author_facet Berra Romani, Roberto
Raqeeb, Abdul
Laforenza, Umberto
Scaffino, Manuela Federica
Moccia, Francesco
Avelino-Cruz, Josè Everardo
Oldani, Amanda
Coltrini, Daniela
Milesi, Verónica
Taglietti, Vanni
Tanzi, Franco
author_sort Berra Romani, Roberto
title Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title_short Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title_full Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title_fullStr Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title_full_unstemmed Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
title_sort cardiac microvascular endothelial cells express a functional ca 2+-sensing receptor
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/83531
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