Multiple nuclear pseudogenes of mitochondrial cytochrome b in Ctenomys (Caviomorpha, Rodentia) with either great similarity to or high divergence from the true mitochondrial sequence

A fragment of the mitochondrial cytochrome b gene was studied in 13 species of the South American fossorial rodent Ctenomys using PCR with 'universal' primers and DNA sequencing after cloning: Five different groups of sequences were found, one of which corresponds to the functional mitocho...

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Detalles Bibliográficos
Autores principales: Mirol, Patricia Mónica, Mascheretti, Silvia, Searle, Jeremy B.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Veterinarias
Ctenomys
Cytochrome b
Mitochondrial DNA
Nuclear pseudogenes
numtDNA
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83502
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83502
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Ctenomys
Cytochrome b
Mitochondrial DNA
Nuclear pseudogenes
numtDNA
spellingShingle Ciencias Veterinarias
Ctenomys
Cytochrome b
Mitochondrial DNA
Nuclear pseudogenes
numtDNA
Mirol, Patricia Mónica
Mascheretti, Silvia
Searle, Jeremy B.
Multiple nuclear pseudogenes of mitochondrial cytochrome b in Ctenomys (Caviomorpha, Rodentia) with either great similarity to or high divergence from the true mitochondrial sequence
topic_facet Ciencias Veterinarias
Ctenomys
Cytochrome b
Mitochondrial DNA
Nuclear pseudogenes
numtDNA
description A fragment of the mitochondrial cytochrome b gene was studied in 13 species of the South American fossorial rodent Ctenomys using PCR with 'universal' primers and DNA sequencing after cloning: Five different groups of sequences were found, one of which corresponds to the functional mitochondrial gene (mt). The other four groups (A, B, C and D) were believed to be nuclear pseudogenes. Sequences A-C were highly divergent from the mt sequences and included substitutions, deletions and insertions such that they could not possibly have coded a functional protein. They all shared a common insertion between positions 15055 and 15056 suggestive of a common origin, although the A, B and C sequences otherwise differed greatly from each other. The D sequences also could not have been functional on the basis of nucleotide sequence, but the differences with the mt sequences were far more subtle and in a more limited study the D sequences could easily have been classified as a true mtDNA sequence. It is suggested that there were two transfers of the cytochrome b gene from the mitochondrion to the nucleus; the first leading to sequences A-C and the second to the D sequence. Subsequent to transfer, a sequence of duplications within the nucleus appears to have generated the full range of pseudogenes that are observed. This study adds to other recent observations suggesting the frequent transfer of mtDNA sequences to the nucleus and reinforces the necessity of great care in interpreting PCR-generated sequences, particularly those produced with universal primers. There are now data from several species of mammals and birds relating to PCR-generated nuclear copies of cytochrome b, which we review.
format Articulo
Articulo
author Mirol, Patricia Mónica
Mascheretti, Silvia
Searle, Jeremy B.
author_facet Mirol, Patricia Mónica
Mascheretti, Silvia
Searle, Jeremy B.
author_sort Mirol, Patricia Mónica
title Multiple nuclear pseudogenes of mitochondrial cytochrome b in Ctenomys (Caviomorpha, Rodentia) with either great similarity to or high divergence from the true mitochondrial sequence
title_short Multiple nuclear pseudogenes of mitochondrial cytochrome b in Ctenomys (Caviomorpha, Rodentia) with either great similarity to or high divergence from the true mitochondrial sequence
title_full Multiple nuclear pseudogenes of mitochondrial cytochrome b in Ctenomys (Caviomorpha, Rodentia) with either great similarity to or high divergence from the true mitochondrial sequence
title_fullStr Multiple nuclear pseudogenes of mitochondrial cytochrome b in Ctenomys (Caviomorpha, Rodentia) with either great similarity to or high divergence from the true mitochondrial sequence
title_full_unstemmed Multiple nuclear pseudogenes of mitochondrial cytochrome b in Ctenomys (Caviomorpha, Rodentia) with either great similarity to or high divergence from the true mitochondrial sequence
title_sort multiple nuclear pseudogenes of mitochondrial cytochrome b in ctenomys (caviomorpha, rodentia) with either great similarity to or high divergence from the true mitochondrial sequence
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/83502
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AT mascherettisilvia multiplenuclearpseudogenesofmitochondrialcytochromebinctenomyscaviomorpharodentiawitheithergreatsimilaritytoorhighdivergencefromthetruemitochondrialsequence
AT searlejeremyb multiplenuclearpseudogenesofmitochondrialcytochromebinctenomyscaviomorpharodentiawitheithergreatsimilaritytoorhighdivergencefromthetruemitochondrialsequence
bdutipo_str Repositorios
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