GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules but is dispensable for the sinorhizobium meliloti-alfalfa symbiotic interaction

The guaB mutant strain Rhizobium tropici CIAT8999-10T is defective in symbiosis with common bean, forming nodules that lack rhizobial content. In order to investigate the timing of the guaB requirement during the nodule formation on the host common bean by the strain CIAT899-10.T, we constructed gen...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Collavino, Mónica Mariana, Riccillo, Pablo Miguel, Grasso, Daniel Horacio, Crespi, M., Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Biología
Bioquímica
Nodule development
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83482
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83482
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bioquímica
Nodule development
spellingShingle Biología
Bioquímica
Nodule development
Collavino, Mónica Mariana
Riccillo, Pablo Miguel
Grasso, Daniel Horacio
Crespi, M.
Aguilar, Orlando Mario
GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules but is dispensable for the sinorhizobium meliloti-alfalfa symbiotic interaction
topic_facet Biología
Bioquímica
Nodule development
description The guaB mutant strain Rhizobium tropici CIAT8999-10T is defective in symbiosis with common bean, forming nodules that lack rhizobial content. In order to investigate the timing of the guaB requirement during the nodule formation on the host common bean by the strain CIAT899-10.T, we constructed gene fusions in which the guaB gene is expressed under the control of the symbiotic promoters nodA, bacA, and nifH. Our data indicated that the guaB is required from the early stages of nodulation because full recovery of the wild-type phenotype was accomplished by the nodA-guaB fusion. In addition, we have constructed a guaB mutant derived from Sinorhizobium meliloti 1021, and shown that, unlike R. tropici, the guaB S. meliloti mutant is auxotrophic for guanine and induces wild-type nodules on alfalfa and Medicago truncatula. The guaB R. tropici mutant also is defective in its symbiosis with Macroptilium atropurpureum and Vigna unguiculata but normal with Leucaena leucocephala. These results show that the requirement of the rhizobial guaB for symbiosis is found to be associated with host plants that form determinate type of nodules.
format Articulo
Articulo
author Collavino, Mónica Mariana
Riccillo, Pablo Miguel
Grasso, Daniel Horacio
Crespi, M.
Aguilar, Orlando Mario
author_facet Collavino, Mónica Mariana
Riccillo, Pablo Miguel
Grasso, Daniel Horacio
Crespi, M.
Aguilar, Orlando Mario
author_sort Collavino, Mónica Mariana
title GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules but is dispensable for the sinorhizobium meliloti-alfalfa symbiotic interaction
title_short GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules but is dispensable for the sinorhizobium meliloti-alfalfa symbiotic interaction
title_full GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules but is dispensable for the sinorhizobium meliloti-alfalfa symbiotic interaction
title_fullStr GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules but is dispensable for the sinorhizobium meliloti-alfalfa symbiotic interaction
title_full_unstemmed GuaB activity is required in Rhizobium tropici during the early stages of nodulation of determinate nodules but is dispensable for the sinorhizobium meliloti-alfalfa symbiotic interaction
title_sort guab activity is required in rhizobium tropici during the early stages of nodulation of determinate nodules but is dispensable for the sinorhizobium meliloti-alfalfa symbiotic interaction
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/83482
work_keys_str_mv AT collavinomonicamariana guabactivityisrequiredinrhizobiumtropiciduringtheearlystagesofnodulationofdeterminatenodulesbutisdispensableforthesinorhizobiummelilotialfalfasymbioticinteraction
AT riccillopablomiguel guabactivityisrequiredinrhizobiumtropiciduringtheearlystagesofnodulationofdeterminatenodulesbutisdispensableforthesinorhizobiummelilotialfalfasymbioticinteraction
AT grassodanielhoracio guabactivityisrequiredinrhizobiumtropiciduringtheearlystagesofnodulationofdeterminatenodulesbutisdispensableforthesinorhizobiummelilotialfalfasymbioticinteraction
AT crespim guabactivityisrequiredinrhizobiumtropiciduringtheearlystagesofnodulationofdeterminatenodulesbutisdispensableforthesinorhizobiummelilotialfalfasymbioticinteraction
AT aguilarorlandomario guabactivityisrequiredinrhizobiumtropiciduringtheearlystagesofnodulationofdeterminatenodulesbutisdispensableforthesinorhizobiummelilotialfalfasymbioticinteraction
bdutipo_str Repositorios
_version_ 1764820488662548483

Ejemplares similares