Evaluation of an indirect enzyme-linked immunosorbent assay for routine screening of Theiler's murine encephalomyelitis virus antibodies in mice colonies

The current study demonstrates the ability of an indirect enzyme-linked immunosorbent assay (iELISA) to detect antibodies against Theiler's murine encephalomyelitis virus in mice colonies. The antigen was produced from infected baby hamster kidney (BHK)-21 cells and treated with 1% Nonidet P40...

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Autores principales: Laborde, Juan Martín, Carbone, Cecilia, Corva, Santiago Gerardo, Galosi, Cecilia Mónica
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Ciencias Veterinarias
Diagnosis
Enzyme-linked immunosorbent assay
Theiler's murine encephalomyelitis virus
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83476
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83476
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Diagnosis
Enzyme-linked immunosorbent assay
Theiler's murine encephalomyelitis virus
spellingShingle Ciencias Veterinarias
Diagnosis
Enzyme-linked immunosorbent assay
Theiler's murine encephalomyelitis virus
Laborde, Juan Martín
Carbone, Cecilia
Corva, Santiago Gerardo
Galosi, Cecilia Mónica
Evaluation of an indirect enzyme-linked immunosorbent assay for routine screening of Theiler's murine encephalomyelitis virus antibodies in mice colonies
topic_facet Ciencias Veterinarias
Diagnosis
Enzyme-linked immunosorbent assay
Theiler's murine encephalomyelitis virus
description The current study demonstrates the ability of an indirect enzyme-linked immunosorbent assay (iELISA) to detect antibodies against Theiler's murine encephalomyelitis virus in mice colonies. The antigen was produced from infected baby hamster kidney (BHK)-21 cells and treated with 1% Nonidet P40 in saline buffer. Control antigen was prepared following the same procedure using uninfected BHK-21 cells. The optimal antigen and serum dilutions were established. The reaction was revealed using an anti-mousehorseradish peroxidase conjugate and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid). Optimized iELISA was validated by detection of antibodies in known positive and negative serum samples before testing the samples of unknown status. Performance of the iELISA was compared with the indirect fluorescent antibody test, and the cutoff value was determined by receiver operating curve. Indirect ELISA showed 100% sensitivity, 99.38% specificity, and 97.78% predictive positive value. The antigen used is easy to produce, and no special equipment is required. The iELISA developed is simple and provides a rapid and less costly tool for diagnosis and research.
format Articulo
Articulo
author Laborde, Juan Martín
Carbone, Cecilia
Corva, Santiago Gerardo
Galosi, Cecilia Mónica
author_facet Laborde, Juan Martín
Carbone, Cecilia
Corva, Santiago Gerardo
Galosi, Cecilia Mónica
author_sort Laborde, Juan Martín
title Evaluation of an indirect enzyme-linked immunosorbent assay for routine screening of Theiler's murine encephalomyelitis virus antibodies in mice colonies
title_short Evaluation of an indirect enzyme-linked immunosorbent assay for routine screening of Theiler's murine encephalomyelitis virus antibodies in mice colonies
title_full Evaluation of an indirect enzyme-linked immunosorbent assay for routine screening of Theiler's murine encephalomyelitis virus antibodies in mice colonies
title_fullStr Evaluation of an indirect enzyme-linked immunosorbent assay for routine screening of Theiler's murine encephalomyelitis virus antibodies in mice colonies
title_full_unstemmed Evaluation of an indirect enzyme-linked immunosorbent assay for routine screening of Theiler's murine encephalomyelitis virus antibodies in mice colonies
title_sort evaluation of an indirect enzyme-linked immunosorbent assay for routine screening of theiler's murine encephalomyelitis virus antibodies in mice colonies
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/83476
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