Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production

We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for...

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Detalles Bibliográficos
Autores principales: Frungieri, Mónica B., Gonzalez Calvar, Silvia I., Parborell, Fernanda, Albrecht, Martin, Mayerhofer, Artur, Calandra, Ricardo Saúl
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Bioquímica
Biología
Cyclooxygenase-2
Prostaglandin F2
Leydig cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83449
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83449
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Biología
Cyclooxygenase-2
Prostaglandin F2
Leydig cells
spellingShingle Bioquímica
Biología
Cyclooxygenase-2
Prostaglandin F2
Leydig cells
Frungieri, Mónica B.
Gonzalez Calvar, Silvia I.
Parborell, Fernanda
Albrecht, Martin
Mayerhofer, Artur
Calandra, Ricardo Saúl
Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
topic_facet Bioquímica
Biología
Cyclooxygenase-2
Prostaglandin F2
Leydig cells
description We have previously found that cyclooxygenase-2 (COX-2), a key enzyme in the biosynthesis of prostaglandins (PGs), is present in the testicular interstitial cells of infertile men, whereas it is absent in human testes with no evident morphological changes or abnormalities. To find an animal model for further investigating COX-2 and its role in testicular steroidogenesis, we screened testes from adult species ranging from mice to monkeys. By using immunohistochemical assays, we found COX-2 expression only in Leydig cells of the reproductively active (peripubertal, pubertal, and adult) seasonal breeder Syrian hamster. COX-2 expression in hamster Leydig cells was confirmed by RT-PCR. In contrast, COX-1 expression was not detected in hamster testes. Because COX-2 expression implies PG synthesis, we investigated the effect of various PGs on testosterone production and found that PGF2α stood out because it significantly reduced human chorionic gonadotropin-stimulated testosterone release from isolated hamster Leydig cells in a dose-dependent manner. This mechanism involves a decreased expression of testicular steroidogenic acute regulatory protein and 17β-hydroxysteroid dehydrogenase. Testicular concentration and content of PGF2α in reproductively active hamsters as well as production of PGF2α from isolated hamster Leydig cells were also determined. Moreover, PGF2α receptors were localized in Leydig cells of hamsters and testicular biopsies from patients with Sertoli cell only and germ arrest syndromes. Thus, in this study, we described a COX-2-initiated pathway that via PGF2α production, PGF2α receptors, steroidogenic acute regulatory protein, and 17β-hydroxysteroid dehydrogenase represents a physiological local inhibitory system of human chorionic gonadotropin-stimulated testosterone production in the Syrian hamster testes.
format Articulo
Articulo
author Frungieri, Mónica B.
Gonzalez Calvar, Silvia I.
Parborell, Fernanda
Albrecht, Martin
Mayerhofer, Artur
Calandra, Ricardo Saúl
author_facet Frungieri, Mónica B.
Gonzalez Calvar, Silvia I.
Parborell, Fernanda
Albrecht, Martin
Mayerhofer, Artur
Calandra, Ricardo Saúl
author_sort Frungieri, Mónica B.
title Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title_short Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title_full Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title_fullStr Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title_full_unstemmed Cyclooxygenase-2 and prostaglandin F2α in syrian hamster leydig cells : Inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
title_sort cyclooxygenase-2 and prostaglandin f2α in syrian hamster leydig cells : inhibitory role on luteinizing hormone/human chorionic gonadotropin-stimulated testosterone production
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/83449
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