Simultaneous determination of cortisone and cortisol in serum by HPLC-DAD and application for pharmacokinetics

To develop a high performance liquid chromatography method for the simultaneous determination of cortisone and cortisol in rat serum and apply it for pharmacokinetics. After addition of pirfenidone as internal standard (IS), a liquid-liquid extraction with ethylacetate was employed for the sample pr...

Descripción completa

Detalles Bibliográficos
Autores principales: Qian, Jianchang, Li, Junwei, Li, Wanshu, Wen, Congcong, Sun, Wei, Zhang, Likang, Hu, Guoxin, Nie, Ben, Qiu, Xiangjun
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
cortisone; cortisol; HPLC; pharmacokinetics; serum
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8344
http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_22.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8344
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
cortisone; cortisol; HPLC; pharmacokinetics; serum
spellingShingle Farmacia
cortisone; cortisol; HPLC; pharmacokinetics; serum
Qian, Jianchang
Li, Junwei
Li, Wanshu
Wen, Congcong
Sun, Wei
Zhang, Likang
Hu, Guoxin
Nie, Ben
Qiu, Xiangjun
Simultaneous determination of cortisone and cortisol in serum by HPLC-DAD and application for pharmacokinetics
topic_facet Farmacia
cortisone; cortisol; HPLC; pharmacokinetics; serum
description To develop a high performance liquid chromatography method for the simultaneous determination of cortisone and cortisol in rat serum and apply it for pharmacokinetics. After addition of pirfenidone as internal standard (IS), a liquid-liquid extraction with ethylacetate was employed for the sample preparation. Samples were separated on Zorbax SB-C18 column at 25 ºC using mobile phase consisting of acetonitrile-water-0.1 % trifluoroacetic acid with flow rate of 0.9 mL/min, utilizing DAD detection at 246 nm. Excellent liner relationships of the cortisone and cortisol concentrations were obtained from 50 to 6000 ng/mL, with r = 0.9997, 0.9999 respectively, and the lower limit of quantitation (LLOQ) were both 50 ng/mL. The developed method was successfully applied to pharmacokinetic studies of cortisone and cortisol in rats following single dose of 20 mg/kg via intraperitoneal injection.
format Articulo
Articulo
author Qian, Jianchang
Li, Junwei
Li, Wanshu
Wen, Congcong
Sun, Wei
Zhang, Likang
Hu, Guoxin
Nie, Ben
Qiu, Xiangjun
author_facet Qian, Jianchang
Li, Junwei
Li, Wanshu
Wen, Congcong
Sun, Wei
Zhang, Likang
Hu, Guoxin
Nie, Ben
Qiu, Xiangjun
author_sort Qian, Jianchang
title Simultaneous determination of cortisone and cortisol in serum by HPLC-DAD and application for pharmacokinetics
title_short Simultaneous determination of cortisone and cortisol in serum by HPLC-DAD and application for pharmacokinetics
title_full Simultaneous determination of cortisone and cortisol in serum by HPLC-DAD and application for pharmacokinetics
title_fullStr Simultaneous determination of cortisone and cortisol in serum by HPLC-DAD and application for pharmacokinetics
title_full_unstemmed Simultaneous determination of cortisone and cortisol in serum by HPLC-DAD and application for pharmacokinetics
title_sort simultaneous determination of cortisone and cortisol in serum by hplc-dad and application for pharmacokinetics
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8344
http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_22.pdf
work_keys_str_mv AT qianjianchang simultaneousdeterminationofcortisoneandcortisolinserumbyhplcdadandapplicationforpharmacokinetics
AT lijunwei simultaneousdeterminationofcortisoneandcortisolinserumbyhplcdadandapplicationforpharmacokinetics
AT liwanshu simultaneousdeterminationofcortisoneandcortisolinserumbyhplcdadandapplicationforpharmacokinetics
AT wencongcong simultaneousdeterminationofcortisoneandcortisolinserumbyhplcdadandapplicationforpharmacokinetics
AT sunwei simultaneousdeterminationofcortisoneandcortisolinserumbyhplcdadandapplicationforpharmacokinetics
AT zhanglikang simultaneousdeterminationofcortisoneandcortisolinserumbyhplcdadandapplicationforpharmacokinetics
AT huguoxin simultaneousdeterminationofcortisoneandcortisolinserumbyhplcdadandapplicationforpharmacokinetics
AT nieben simultaneousdeterminationofcortisoneandcortisolinserumbyhplcdadandapplicationforpharmacokinetics
AT qiuxiangjun simultaneousdeterminationofcortisoneandcortisolinserumbyhplcdadandapplicationforpharmacokinetics
bdutipo_str Repositorios
_version_ 1764820488618508293

Ejemplares similares