Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors
The aim of this work was to study the possible relationship between pancreatic duodenal homeobox-1 (Pdx-1) and islet neogenesis-associated protein (INGAP) during induced islet neogenesis. Pregnant hamsters were fed with (S) and without (C) sucrose, and glycemia, insulin secretion <i>in vitro&l...
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Formato: | Articulo |
Lenguaje: | Inglés |
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2003
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/83428 |
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I19-R120-10915-83428 |
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Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
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SEDICI (UNLP) |
language |
Inglés |
topic |
Ciencias Médicas pancreatic duodenal homeobox-1 islet neogenesis-associated protein neogenesis |
spellingShingle |
Ciencias Médicas pancreatic duodenal homeobox-1 islet neogenesis-associated protein neogenesis Gagliardino, Juan José Del Zotto, Héctor Herminio Massa, María Laura Flores, Luis Emilio Borelli, María Inés Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors |
topic_facet |
Ciencias Médicas pancreatic duodenal homeobox-1 islet neogenesis-associated protein neogenesis |
description |
The aim of this work was to study the possible relationship between pancreatic duodenal homeobox-1 (Pdx-1) and islet neogenesis-associated protein (INGAP) during induced islet neogenesis. Pregnant hamsters were fed with (S) and without (C) sucrose, and glycemia, insulin secretion <i>in vitro</i>, and pancreas immunomorphometric parameters were measured in their 7-day-old offspring. S offspring had significantly lower glycemic levels than C animals. Insulin release in response to increasing glucose contrations in the incubation medium (2-16 mM glucose) did not increase in pancreata from either C or S offspring. However, pancreata from S offspring released more insulin than those from C animals. In S offspring, β-cell mass, β-cell replication rate and islet neogenesis increased significantly, with a simultaneous decrease in β-cell apoptotic rate. INGAP- and Pdx-1-positive cell mass also increased in the islets and among acinar and duct cells. We found two subpopulations of Pdx-1 cells: INGAP-positive and INGAP-negative. Pdx-1/INGAP-positive cells did not stain with insulin, glucagon, somatostatin, pancreatic polypeptide, or neurogenin 3 antibodies. The increment of Pdx-1/INGAP-positive cells represented the major contribution to the Pdx-1 cell mass increase. Such increments varied among pancreas subsectors: ductal>insular>extrainsular. Our results suggested that INGAP participates in the regulation of islet neogenesis, and Pdx-1/INGAP-positive cells represent a new stem cell subpopulation at an early stage of development, highly activateable in neogenesis. |
format |
Articulo Articulo |
author |
Gagliardino, Juan José Del Zotto, Héctor Herminio Massa, María Laura Flores, Luis Emilio Borelli, María Inés |
author_facet |
Gagliardino, Juan José Del Zotto, Héctor Herminio Massa, María Laura Flores, Luis Emilio Borelli, María Inés |
author_sort |
Gagliardino, Juan José |
title |
Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors |
title_short |
Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors |
title_full |
Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors |
title_fullStr |
Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors |
title_full_unstemmed |
Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors |
title_sort |
pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: a possible combined marker of activateable pancreatic cell precursors |
publishDate |
2003 |
url |
http://sedici.unlp.edu.ar/handle/10915/83428 |
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bdutipo_str |
Repositorios |
_version_ |
1764820488608022529 |