Development of an immunochemical method to detect <i>Lactobacillus kefir</i>

Kefir is a fermented milk elaborated with kefir grains. It constitutes an ecological system composed by a protein-polysaccharide matrix on which a complex microbiota is naturally immobilized. The objective of this work was to develop an antibody-based method in order to achieve a simple and rapid wa...

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Detalles Bibliográficos
Autores principales: Garrote, Graciela Liliana, Serradell, María de los Ángeles, Abraham, Analía Graciela, Añón, María Cristina, Fossati, Carlos Alberto, De Antoni, Graciela Liliana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Química
ELISA
Immunodetection
Kefir milk
Lactobacilli
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83403
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83403
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
ELISA
Immunodetection
Kefir milk
Lactobacilli
spellingShingle Química
ELISA
Immunodetection
Kefir milk
Lactobacilli
Garrote, Graciela Liliana
Serradell, María de los Ángeles
Abraham, Analía Graciela
Añón, María Cristina
Fossati, Carlos Alberto
De Antoni, Graciela Liliana
Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
topic_facet Química
ELISA
Immunodetection
Kefir milk
Lactobacilli
description Kefir is a fermented milk elaborated with kefir grains. It constitutes an ecological system composed by a protein-polysaccharide matrix on which a complex microbiota is naturally immobilized. The objective of this work was to develop an antibody-based method in order to achieve a simple and rapid way to quantify Lactobacillus kefir in a complex environment. Rabbit antisera against isolated S-layer proteins from L. kefir and L. parakefir were obtained. Both antisera recognized the 21 strains of L. kefir and the three strains of L. parakefir analysed by dot-blot. By indirect ELISA, specific antiserum against L. kefir S-layer showed reactivity against pure cultures of both L. kefir and L. parakefir, but did not react with L. brevis cells. Competitive ELISA allowed quantification of L. kefir at concentrations ranging from 5 × 10 5 to 108 bacteria ml-1, but neither L. parakefir nor L. brevis pure cultures produced inhibition. Through this approach it was possible to detect 108 L. kefir ml-1 in samples of milk fermented for 48 h at 20°C with different kefir grains. Competitive ELISA developed in our laboratory is a suitable method to detect and quantify L. kefir in milk and in consequence it could be applied to analyse the relationship between the composition of microflora and probiotic and technological characteristics of the products.
format Articulo
Articulo
author Garrote, Graciela Liliana
Serradell, María de los Ángeles
Abraham, Analía Graciela
Añón, María Cristina
Fossati, Carlos Alberto
De Antoni, Graciela Liliana
author_facet Garrote, Graciela Liliana
Serradell, María de los Ángeles
Abraham, Analía Graciela
Añón, María Cristina
Fossati, Carlos Alberto
De Antoni, Graciela Liliana
author_sort Garrote, Graciela Liliana
title Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title_short Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title_full Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title_fullStr Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title_full_unstemmed Development of an immunochemical method to detect <i>Lactobacillus kefir</i>
title_sort development of an immunochemical method to detect <i>lactobacillus kefir</i>
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/83403
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