Studies on the gonadotropin-releasing activity of thymulin: Changes with age
We assessed the ability of thymulin, a zinc-dependent nonapeptide produced by the thymic epithelial cells, to influence the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from dispersed anterior pituitary (AP) cells from young, adult, and senescent female rats. Perifusion...
| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2000
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/83368 |
| Aporte de: |
| id |
I19-R120-10915-83368 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Médicas Gonadotropinas |
| spellingShingle |
Ciencias Médicas Gonadotropinas Brown, Oscar Alfredo Sosa, Yolanda Elena Dardenne, Mireille Pléau, Jean Marie Goya, Rodolfo Gustavo Studies on the gonadotropin-releasing activity of thymulin: Changes with age |
| topic_facet |
Ciencias Médicas Gonadotropinas |
| description |
We assessed the ability of thymulin, a zinc-dependent nonapeptide produced by the thymic epithelial cells, to influence the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from dispersed anterior pituitary (AP) cells from young, adult, and senescent female rats. Perifusion of young and senescent AP cells with thymulin doses of 10-6 to 10-5 M gave a significant stimulatory response for LH but not FSH. Gonadotropin release was always lower in the senescent cells. AP cells from both age groups incubated with 10-8 to 10-3 M thymulin showed a time- and dose-dependent response for both gonadotropins, with a maximal stimulation at 10-7 M. Preincubation of thymulin with an antithymulin serum completely quenched the secretagogue activity of the hormone. Coincubation of thymulin with the secretagogue gonadotropin-releasing hormone (GnRH) revealed a synergistic effect on LH release and an additive effect on the release of FSH. The calcium chelator EGTA blocked the gonadotropin-releasing activity of thymulin in AP cells. The cAMP enhancers, caffeine, NaF, and forskolin significantly increased the thymulin-stimulated release of gonadotropins. The inositol phosphate enhancer LiCl potentiated the action of thymulin on gonadotropins. It is concluded that the gonadotropin-releasing activity documented here for thymulin is an age- and receptor-dependent effect mediated in part by calcium, cAMP, and inositol phosphates. |
| format |
Articulo Articulo |
| author |
Brown, Oscar Alfredo Sosa, Yolanda Elena Dardenne, Mireille Pléau, Jean Marie Goya, Rodolfo Gustavo |
| author_facet |
Brown, Oscar Alfredo Sosa, Yolanda Elena Dardenne, Mireille Pléau, Jean Marie Goya, Rodolfo Gustavo |
| author_sort |
Brown, Oscar Alfredo |
| title |
Studies on the gonadotropin-releasing activity of thymulin: Changes with age |
| title_short |
Studies on the gonadotropin-releasing activity of thymulin: Changes with age |
| title_full |
Studies on the gonadotropin-releasing activity of thymulin: Changes with age |
| title_fullStr |
Studies on the gonadotropin-releasing activity of thymulin: Changes with age |
| title_full_unstemmed |
Studies on the gonadotropin-releasing activity of thymulin: Changes with age |
| title_sort |
studies on the gonadotropin-releasing activity of thymulin: changes with age |
| publishDate |
2000 |
| url |
http://sedici.unlp.edu.ar/handle/10915/83368 |
| work_keys_str_mv |
AT brownoscaralfredo studiesonthegonadotropinreleasingactivityofthymulinchangeswithage AT sosayolandaelena studiesonthegonadotropinreleasingactivityofthymulinchangeswithage AT dardennemireille studiesonthegonadotropinreleasingactivityofthymulinchangeswithage AT pleaujeanmarie studiesonthegonadotropinreleasingactivityofthymulinchangeswithage AT goyarodolfogustavo studiesonthegonadotropinreleasingactivityofthymulinchangeswithage |
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Repositorios |
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1764820488548253696 |