Studies on the gonadotropin-releasing activity of thymulin: Changes with age

We assessed the ability of thymulin, a zinc-dependent nonapeptide produced by the thymic epithelial cells, to influence the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from dispersed anterior pituitary (AP) cells from young, adult, and senescent female rats. Perifusion...

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Autores principales: Brown, Oscar Alfredo, Sosa, Yolanda Elena, Dardenne, Mireille, Pléau, Jean Marie, Goya, Rodolfo Gustavo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Médicas
Gonadotropinas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83368
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83368
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Gonadotropinas
spellingShingle Ciencias Médicas
Gonadotropinas
Brown, Oscar Alfredo
Sosa, Yolanda Elena
Dardenne, Mireille
Pléau, Jean Marie
Goya, Rodolfo Gustavo
Studies on the gonadotropin-releasing activity of thymulin: Changes with age
topic_facet Ciencias Médicas
Gonadotropinas
description We assessed the ability of thymulin, a zinc-dependent nonapeptide produced by the thymic epithelial cells, to influence the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from dispersed anterior pituitary (AP) cells from young, adult, and senescent female rats. Perifusion of young and senescent AP cells with thymulin doses of 10-6 to 10-5 M gave a significant stimulatory response for LH but not FSH. Gonadotropin release was always lower in the senescent cells. AP cells from both age groups incubated with 10-8 to 10-3 M thymulin showed a time- and dose-dependent response for both gonadotropins, with a maximal stimulation at 10-7 M. Preincubation of thymulin with an antithymulin serum completely quenched the secretagogue activity of the hormone. Coincubation of thymulin with the secretagogue gonadotropin-releasing hormone (GnRH) revealed a synergistic effect on LH release and an additive effect on the release of FSH. The calcium chelator EGTA blocked the gonadotropin-releasing activity of thymulin in AP cells. The cAMP enhancers, caffeine, NaF, and forskolin significantly increased the thymulin-stimulated release of gonadotropins. The inositol phosphate enhancer LiCl potentiated the action of thymulin on gonadotropins. It is concluded that the gonadotropin-releasing activity documented here for thymulin is an age- and receptor-dependent effect mediated in part by calcium, cAMP, and inositol phosphates.
format Articulo
Articulo
author Brown, Oscar Alfredo
Sosa, Yolanda Elena
Dardenne, Mireille
Pléau, Jean Marie
Goya, Rodolfo Gustavo
author_facet Brown, Oscar Alfredo
Sosa, Yolanda Elena
Dardenne, Mireille
Pléau, Jean Marie
Goya, Rodolfo Gustavo
author_sort Brown, Oscar Alfredo
title Studies on the gonadotropin-releasing activity of thymulin: Changes with age
title_short Studies on the gonadotropin-releasing activity of thymulin: Changes with age
title_full Studies on the gonadotropin-releasing activity of thymulin: Changes with age
title_fullStr Studies on the gonadotropin-releasing activity of thymulin: Changes with age
title_full_unstemmed Studies on the gonadotropin-releasing activity of thymulin: Changes with age
title_sort studies on the gonadotropin-releasing activity of thymulin: changes with age
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/83368
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