Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila

The Drosophila genome-sequencing project has revealed a total of seven genes encoding eight eukaryotic initiation factor 4E (eIF4E) isoforms. Four of them (eIF4E-1,2, eIF4E-3, eIF4E-4 and eIF4E-5) share exon/intron structure in their carboxy-terminal part and form a cluster in the genome. All eIF4E...

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Autores principales: Hernández, Greco, Altmann, Michael, Sierra, José Manuel, Urlaub, Henning, Diez del Corral, Ruth, Schwartz, Peter, Rivera Pomar, Rolando Víctor
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Ciencias Exactas
Drosophila
eIF4E isoforms
Initiation of translation
l(3)67Af
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83347
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83347
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Drosophila
eIF4E isoforms
Initiation of translation
l(3)67Af
spellingShingle Ciencias Exactas
Drosophila
eIF4E isoforms
Initiation of translation
l(3)67Af
Hernández, Greco
Altmann, Michael
Sierra, José Manuel
Urlaub, Henning
Diez del Corral, Ruth
Schwartz, Peter
Rivera Pomar, Rolando Víctor
Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila
topic_facet Ciencias Exactas
Drosophila
eIF4E isoforms
Initiation of translation
l(3)67Af
description The Drosophila genome-sequencing project has revealed a total of seven genes encoding eight eukaryotic initiation factor 4E (eIF4E) isoforms. Four of them (eIF4E-1,2, eIF4E-3, eIF4E-4 and eIF4E-5) share exon/intron structure in their carboxy-terminal part and form a cluster in the genome. All eIF4E isoforms bind to the cap (m7GpppN) structure. All of them, except eIF4E-6 and eIF4E-8 were able to interact with Drosophila eIF4G or eIF4E-binding protein (4E-BP). eIF4E-1, eIF4E-2, eIF4E-3, eIF4E-4 and eIF4E-7 rescued a yeast eIF4E-deficient mutant in vivo. Only eIF4E-1 mRNAs and, at a significantly lower level, eIF4E3 and eIF4E-8 are expressed in embryos and throughout the life cycle of the fly. The transcripts of the remaining isoforms were detected from the third instar larvae onwards. This indicates the cap-binding activity relies mostly on eIF4E-1 during embryogenesis. This agrees with the proteomic analysis of the eIF4F complex purified from embryos and with the rescue of l(3)67Af, an embryonic lethal mutant for the eIF4E-1,2 gene, by transgenic expression of eIF4E-1. Overexpression of eIF4E-1 in wild-type embryos and eye imaginal discs results in phenotypic defects in a dose-dependent manner.
format Articulo
Articulo
author Hernández, Greco
Altmann, Michael
Sierra, José Manuel
Urlaub, Henning
Diez del Corral, Ruth
Schwartz, Peter
Rivera Pomar, Rolando Víctor
author_facet Hernández, Greco
Altmann, Michael
Sierra, José Manuel
Urlaub, Henning
Diez del Corral, Ruth
Schwartz, Peter
Rivera Pomar, Rolando Víctor
author_sort Hernández, Greco
title Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila
title_short Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila
title_full Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila
title_fullStr Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila
title_full_unstemmed Functional analysis of seven genes encoding eight translation initiation factor 4E (eIF4E) isoforms in Drosophila
title_sort functional analysis of seven genes encoding eight translation initiation factor 4e (eif4e) isoforms in drosophila
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/83347
work_keys_str_mv AT hernandezgreco functionalanalysisofsevengenesencodingeighttranslationinitiationfactor4eeif4eisoformsindrosophila
AT altmannmichael functionalanalysisofsevengenesencodingeighttranslationinitiationfactor4eeif4eisoformsindrosophila
AT sierrajosemanuel functionalanalysisofsevengenesencodingeighttranslationinitiationfactor4eeif4eisoformsindrosophila
AT urlaubhenning functionalanalysisofsevengenesencodingeighttranslationinitiationfactor4eeif4eisoformsindrosophila
AT diezdelcorralruth functionalanalysisofsevengenesencodingeighttranslationinitiationfactor4eeif4eisoformsindrosophila
AT schwartzpeter functionalanalysisofsevengenesencodingeighttranslationinitiationfactor4eeif4eisoformsindrosophila
AT riverapomarrolandovictor functionalanalysisofsevengenesencodingeighttranslationinitiationfactor4eeif4eisoformsindrosophila
bdutipo_str Repositorios
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