SPARC endogenous level, rather than fibroblast-produced SPARC or stroma reorganization induced by SPARC, is responsible for melanoma cell growth

SPARC (secreted protein acidic and rich in cysteine) is a matricellular protein whose overexpression in malignant or tumor-stromal cells is often associated with increased aggressiveness and bad prognosis in a wide range of human cancer types, particularly melanoma. We established the impact that ch...

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Detalles Bibliográficos
Autores principales:	Prada, Federico, Benedetti, Lorena G., Bravo, Alicia I., Alvarez, Mariano J., Carbone, Cecilia, Podhajcer, Osvaldo L.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2007
Materias:	Ciencias Médicas Ciencias Veterinarias SPARC human cancer Melanoma
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/83339
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-83339
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas Ciencias Veterinarias SPARC human cancer Melanoma
spellingShingle	Ciencias Médicas Ciencias Veterinarias SPARC human cancer Melanoma Prada, Federico Benedetti, Lorena G. Bravo, Alicia I. Alvarez, Mariano J. Carbone, Cecilia Podhajcer, Osvaldo L. SPARC endogenous level, rather than fibroblast-produced SPARC or stroma reorganization induced by SPARC, is responsible for melanoma cell growth
topic_facet	Ciencias Médicas Ciencias Veterinarias SPARC human cancer Melanoma
description	SPARC (secreted protein acidic and rich in cysteine) is a matricellular protein whose overexpression in malignant or tumor-stromal cells is often associated with increased aggressiveness and bad prognosis in a wide range of human cancer types, particularly melanoma. We established the impact that changes in the level of SPARC produced by malignant cells and neighboring stromal cells have on melanoma growth. Melanoma cell growth in monolayer was only slightly affected by changes in SPARC levels. However, melanoma growth in spheroids was strongly inhibited upon SPARC hyperexpression and conversely enhanced when SPARC expression was downregulated. Interestingly, SPARC overexpression in neighboring fibroblasts had no effect on spheroid growth irrespective of SPARC levels expressed by the melanoma cells, themselves. Downregulation of SPARC expression in melanoma cells induced their rejection <i>in vivo</i> through a mechanism mediated exclusively by host polymorphonuclear cells. On the other hand, SPARC hyperexpression enhanced vascular density, collagen deposition, and fibroblast recruitment in the surrounding stroma without affecting melanoma growth. In agreement with the <i>in vitro</i> data, overexpression of SPARC in co-injected fibroblasts did not affect melanoma growth <i>in vivo</i>. All the data indicate that melanoma growth is not subject to regulation by exogenous SPARC, nor by stromal organization, but only by SPARC levels produced by the malignant cells themselves.
format	Articulo Articulo
author	Prada, Federico Benedetti, Lorena G. Bravo, Alicia I. Alvarez, Mariano J. Carbone, Cecilia Podhajcer, Osvaldo L.
author_facet	Prada, Federico Benedetti, Lorena G. Bravo, Alicia I. Alvarez, Mariano J. Carbone, Cecilia Podhajcer, Osvaldo L.
author_sort	Prada, Federico
title	SPARC endogenous level, rather than fibroblast-produced SPARC or stroma reorganization induced by SPARC, is responsible for melanoma cell growth
title_short	SPARC endogenous level, rather than fibroblast-produced SPARC or stroma reorganization induced by SPARC, is responsible for melanoma cell growth
title_full	SPARC endogenous level, rather than fibroblast-produced SPARC or stroma reorganization induced by SPARC, is responsible for melanoma cell growth
title_fullStr	SPARC endogenous level, rather than fibroblast-produced SPARC or stroma reorganization induced by SPARC, is responsible for melanoma cell growth
title_full_unstemmed	SPARC endogenous level, rather than fibroblast-produced SPARC or stroma reorganization induced by SPARC, is responsible for melanoma cell growth
title_sort	sparc endogenous level, rather than fibroblast-produced sparc or stroma reorganization induced by sparc, is responsible for melanoma cell growth
publishDate	2007
url	http://sedici.unlp.edu.ar/handle/10915/83339
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bdutipo_str	Repositorios
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SPARC endogenous level, rather than fibroblast-produced SPARC or stroma reorganization induced by SPARC, is responsible for melanoma cell growth

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