Gradient elution LC-ESI-MS determination of tramadol in rat plasma

A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of tramadol in rat plasma using one-step protein precipitation was developed. After addition of ketamine as internal standard (IS), protein precipitation by acetonitrile was used as sampl...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lin, Chongliang, Lin, Guanyang, Zhou, Ziye, Hu, Lufeng, Wang, Xianqin, Ma Jianshe
Formato: Articulo
Lenguaje:Español
Publicado: 2011
Materias:
Farmacia
gradient elution; LC-ESI-MS; rat plasma; tramadol
Cromatografía Liquida
Espectrometría
Plasma
Tramadol
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8330
http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_7.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8330
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Farmacia
gradient elution; LC-ESI-MS; rat plasma; tramadol
Cromatografía Liquida
Espectrometría
Plasma
Tramadol
spellingShingle Farmacia
gradient elution; LC-ESI-MS; rat plasma; tramadol
Cromatografía Liquida
Espectrometría
Plasma
Tramadol
Lin, Chongliang
Lin, Guanyang
Zhou, Ziye
Hu, Lufeng
Wang, Xianqin
Ma Jianshe
Gradient elution LC-ESI-MS determination of tramadol in rat plasma
topic_facet Farmacia
gradient elution; LC-ESI-MS; rat plasma; tramadol
Cromatografía Liquida
Espectrometría
Plasma
Tramadol
description A sensitive and simple liquid chromatography/electrospray mass spectrometry (LC-ESI-MS) method for determination of tramadol in rat plasma using one-step protein precipitation was developed. After addition of ketamine as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 mm × 50 mm, 3.5 μm) column with methanol-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 264.0 for tramadol and m/z 237.8 for the IS. Calibration plots were linear over the range of 5-500 ng/mL for tramadol in rat plasma. Lower limit of quantification (LLOQ) for tramadol was 5 ng/mL. Mean recovery of tramadol from plasma was in the range 92.8 %-97.4 %. RSD of intra-day and inter-day precision were both less than 10 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of tramadol in rat plasma.
format Articulo
Articulo
author Lin, Chongliang
Lin, Guanyang
Zhou, Ziye
Hu, Lufeng
Wang, Xianqin
Ma Jianshe
author_facet Lin, Chongliang
Lin, Guanyang
Zhou, Ziye
Hu, Lufeng
Wang, Xianqin
Ma Jianshe
author_sort Lin, Chongliang
title Gradient elution LC-ESI-MS determination of tramadol in rat plasma
title_short Gradient elution LC-ESI-MS determination of tramadol in rat plasma
title_full Gradient elution LC-ESI-MS determination of tramadol in rat plasma
title_fullStr Gradient elution LC-ESI-MS determination of tramadol in rat plasma
title_full_unstemmed Gradient elution LC-ESI-MS determination of tramadol in rat plasma
title_sort gradient elution lc-esi-ms determination of tramadol in rat plasma
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8330
http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_7.pdf
work_keys_str_mv AT linchongliang gradientelutionlcesimsdeterminationoftramadolinratplasma
AT linguanyang gradientelutionlcesimsdeterminationoftramadolinratplasma
AT zhouziye gradientelutionlcesimsdeterminationoftramadolinratplasma
AT hulufeng gradientelutionlcesimsdeterminationoftramadolinratplasma
AT wangxianqin gradientelutionlcesimsdeterminationoftramadolinratplasma
AT majianshe gradientelutionlcesimsdeterminationoftramadolinratplasma
bdutipo_str Repositorios
_version_ 1764820488476950530

Ejemplares similares