Whole-genome analysis of Leptospira interrogans to identify potential vaccine candidates against leptospirosis

Leptospirosis is an important global human and veterinary health problem. Humans can be infected by exposure to chronically infected animals and their environment. An important focus of the current leptospiral research is the identification of outer membrane proteins (OMPs). Due to their location, l...

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Detalles Bibliográficos
Autores principales:	Gamberini, Marcia, Gómez, Ricardo Martín, Atzingen, Marina V., Martins, Elizabeth A. L., Vasconcellos, Silvio A., Romero, Eliete C., Leite, Luciana C. C., Ho, Paulo L., Nascimento, Ana L. T. O.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2005
Materias:	Ciencias Exactas Cloning and expression Leptospira interrogans Leptospirosis Surface protein Vaccine
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/83275
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-83275
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Cloning and expression Leptospira interrogans Leptospirosis Surface protein Vaccine
spellingShingle	Ciencias Exactas Cloning and expression Leptospira interrogans Leptospirosis Surface protein Vaccine Gamberini, Marcia Gómez, Ricardo Martín Atzingen, Marina V. Martins, Elizabeth A. L. Vasconcellos, Silvio A. Romero, Eliete C. Leite, Luciana C. C. Ho, Paulo L. Nascimento, Ana L. T. O. Whole-genome analysis of Leptospira interrogans to identify potential vaccine candidates against leptospirosis
topic_facet	Ciencias Exactas Cloning and expression Leptospira interrogans Leptospirosis Surface protein Vaccine
description	Leptospirosis is an important global human and veterinary health problem. Humans can be infected by exposure to chronically infected animals and their environment. An important focus of the current leptospiral research is the identification of outer membrane proteins (OMPs). Due to their location, leptospiral OMPs are likely to be relevant in host-pathogen interactions, hence their potential ability to stimulate heterologous immunity. The existing whole-genome sequence of <i>Leptospira interrogans</i> serovar Copenhageni offers a unique opportunity to search for cell surface proteins. Predicted genes encoding potential surface proteins were amplified from genomic DNA by PCR methodology and cloned into an <i>Escherichia coli</i> expression system. The partially purified recombinant proteins were probed by Western blotting with sera from human patients diagnosed with leptospirosis. Sixteen proteins, out of a hundred tested, were recognized by antibodies present in human sera. Four of these proteins were conserved among eight serovars of <i>L. interrogans</i> and absent in the non-pathogenic <i>Leptospira biflexa</i>. These proteins might be useful for the diagnosis of the disease as well as potential vaccine candidates.
format	Articulo Articulo
author	Gamberini, Marcia Gómez, Ricardo Martín Atzingen, Marina V. Martins, Elizabeth A. L. Vasconcellos, Silvio A. Romero, Eliete C. Leite, Luciana C. C. Ho, Paulo L. Nascimento, Ana L. T. O.
author_facet	Gamberini, Marcia Gómez, Ricardo Martín Atzingen, Marina V. Martins, Elizabeth A. L. Vasconcellos, Silvio A. Romero, Eliete C. Leite, Luciana C. C. Ho, Paulo L. Nascimento, Ana L. T. O.
author_sort	Gamberini, Marcia
title	Whole-genome analysis of Leptospira interrogans to identify potential vaccine candidates against leptospirosis
title_short	Whole-genome analysis of Leptospira interrogans to identify potential vaccine candidates against leptospirosis
title_full	Whole-genome analysis of Leptospira interrogans to identify potential vaccine candidates against leptospirosis
title_fullStr	Whole-genome analysis of Leptospira interrogans to identify potential vaccine candidates against leptospirosis
title_full_unstemmed	Whole-genome analysis of Leptospira interrogans to identify potential vaccine candidates against leptospirosis
title_sort	whole-genome analysis of leptospira interrogans to identify potential vaccine candidates against leptospirosis
publishDate	2005
url	http://sedici.unlp.edu.ar/handle/10915/83275
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Whole-genome analysis of Leptospira interrogans to identify potential vaccine candidates against leptospirosis

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