Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation

Qualitative and quantitative changes of microbial communities in soil microcosms during bioremediation were determined throughout one year. The soil was contaminated with 0%, 2.5%, 5%, 10% (wt/wt) of petrochemical sludge containing polynuclear aromatic hydrocarbons. We analyzed the hydrocarbon conce...

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Autores principales: Del Panno, María Teresa, Morelli, Irma Susana, Engelen, Bert, Berthe Corti, Luise
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Ciencias Exactas
Bioremediation
Hydrocarbon degradation
Soil bacterial community
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83273
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83273
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bioremediation
Hydrocarbon degradation
Soil bacterial community
spellingShingle Ciencias Exactas
Bioremediation
Hydrocarbon degradation
Soil bacterial community
Del Panno, María Teresa
Morelli, Irma Susana
Engelen, Bert
Berthe Corti, Luise
Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation
topic_facet Ciencias Exactas
Bioremediation
Hydrocarbon degradation
Soil bacterial community
description Qualitative and quantitative changes of microbial communities in soil microcosms during bioremediation were determined throughout one year. The soil was contaminated with 0%, 2.5%, 5%, 10% (wt/wt) of petrochemical sludge containing polynuclear aromatic hydrocarbons. We analyzed the hydrocarbon concentration in the microcosms, the number of cultivable bacteria using CFU and most probable number assays, the community structure using denaturing gradient gel electrophoresis, and the metabolic activity of soil using dehydrogenase activity and substrate-induced respiration assays. After one year of treatment, the chemical analysis suggested that the hydrocarbon elimination process was over. The biological analysis, however, showed that the contaminated microcosms suffered under long-term disturbance. The number of heterotrophic bacteria that increased after sludge addition (up to 10<SUP>8</SUP>-10<SUP>9</SUP> cells ml<SUP>-1</SUP>) has not returned to the level of the control soil (2-6 × 10<SUP>7</SUP> cells ml<SUP>-1</SUP>). The community structure in the contaminated soils differed considerably from that in the control. The substrate-induced respiration of the contaminated soils was significantly lower (≈10-fold) and the dehydrogenase activity was significantly higher (20-40-fold) compared to the control. Changes in the community structure of soils depended on the amount of added sludge. The species, which were predominant in the sludge community, could not be detected in the contaminated soils.
format Articulo
Articulo
author Del Panno, María Teresa
Morelli, Irma Susana
Engelen, Bert
Berthe Corti, Luise
author_facet Del Panno, María Teresa
Morelli, Irma Susana
Engelen, Bert
Berthe Corti, Luise
author_sort Del Panno, María Teresa
title Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation
title_short Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation
title_full Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation
title_fullStr Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation
title_full_unstemmed Effect of petrochemical sludge concentrations on microbial communities during soil bioremediation
title_sort effect of petrochemical sludge concentrations on microbial communities during soil bioremediation
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/83273
work_keys_str_mv AT delpannomariateresa effectofpetrochemicalsludgeconcentrationsonmicrobialcommunitiesduringsoilbioremediation
AT morelliirmasusana effectofpetrochemicalsludgeconcentrationsonmicrobialcommunitiesduringsoilbioremediation
AT engelenbert effectofpetrochemicalsludgeconcentrationsonmicrobialcommunitiesduringsoilbioremediation
AT berthecortiluise effectofpetrochemicalsludgeconcentrationsonmicrobialcommunitiesduringsoilbioremediation
bdutipo_str Repositorios
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