Amphotericin B plasma monitoring for one burn child using high-performance liquid chromatography

A bioanalytical micromethod was described for the quantification of amphotericin B in plasma by HPLC. The method showed high absolute recovery, good linearity (0.1-10.0 μg/mL, r2 = 0.999), sensitivity (limits of quantification: 0.1 μg/mL), and acceptable stability. Inter/intraday precisions were 6.8...

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Autores principales:	Sanches Giraud, Cristina, Gomez, David S., Campos, Edvaldo V., Azevedo, Rodrigo P. de, Despinoy, Alessandra, Perez, Grazziela S., Ferreira, Marcus C., Santos, Silvia R. C. J.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia Macrólidos amphotericin B; burn child; drug plasma monitoring; HPLC-UV; pharmacokinetics Cromatografía Plasma
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8325 http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_2.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8325
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia Macrólidos amphotericin B; burn child; drug plasma monitoring; HPLC-UV; pharmacokinetics Cromatografía Plasma
spellingShingle	Farmacia Macrólidos amphotericin B; burn child; drug plasma monitoring; HPLC-UV; pharmacokinetics Cromatografía Plasma Sanches Giraud, Cristina Gomez, David S. Campos, Edvaldo V. Azevedo, Rodrigo P. de Despinoy, Alessandra Perez, Grazziela S. Ferreira, Marcus C. Santos, Silvia R. C. J. Amphotericin B plasma monitoring for one burn child using high-performance liquid chromatography
topic_facet	Farmacia Macrólidos amphotericin B; burn child; drug plasma monitoring; HPLC-UV; pharmacokinetics Cromatografía Plasma
description	A bioanalytical micromethod was described for the quantification of amphotericin B in plasma by HPLC. The method showed high absolute recovery, good linearity (0.1-10.0 μg/mL, r2 = 0.999), sensitivity (limits of quantification: 0.1 μg/mL), and acceptable stability. Inter/intraday precisions were 6.8 %/2.3 % and mean accuracy was 94.3 %. The method was applied to plasma monitoring of one burn child, 3 years old, 25 kg, thermal injury (18 % total burn surface area - TBSA). Amphotericin B (1 mg/kg) was prescribed from 24th to 35th day of the accident and plasma monitoring and pharmacokinetics was performed by serial blood collections on 27th and 35th days post burn. Plasma concentrations obtained were respectively 0.7 μg/mL and 1.2 μg/mL. Pharmacokinetics at both periods (27th vs 35th day) also was compared: 13.8 vs 14.3 h (t1/2β ); 0.5 vs 0.3 mL/min.kg (CLT) and 0.65 vs 0.38 L/kg (Vdss ). In conclusion, drug plasma monitoring by HPLC was quite useful to guarantee low risk and drug efficacy in a paediatric burn patient.
format	Articulo Articulo
author	Sanches Giraud, Cristina Gomez, David S. Campos, Edvaldo V. Azevedo, Rodrigo P. de Despinoy, Alessandra Perez, Grazziela S. Ferreira, Marcus C. Santos, Silvia R. C. J.
author_facet	Sanches Giraud, Cristina Gomez, David S. Campos, Edvaldo V. Azevedo, Rodrigo P. de Despinoy, Alessandra Perez, Grazziela S. Ferreira, Marcus C. Santos, Silvia R. C. J.
author_sort	Sanches Giraud, Cristina
title	Amphotericin B plasma monitoring for one burn child using high-performance liquid chromatography
title_short	Amphotericin B plasma monitoring for one burn child using high-performance liquid chromatography
title_full	Amphotericin B plasma monitoring for one burn child using high-performance liquid chromatography
title_fullStr	Amphotericin B plasma monitoring for one burn child using high-performance liquid chromatography
title_full_unstemmed	Amphotericin B plasma monitoring for one burn child using high-performance liquid chromatography
title_sort	amphotericin b plasma monitoring for one burn child using high-performance liquid chromatography
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8325 http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_2.pdf
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bdutipo_str	Repositorios
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Amphotericin B plasma monitoring for one burn child using high-performance liquid chromatography

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