Detection of equine herpesvirus 1 genome 1B in Argentina

To determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory s...

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Detalles Bibliográficos
Autores principales: Martínez, J. P., Martín Ocampos, Giselle Paula, Fernandez, L. C., Fuentealba, Nadia Analía, Cid de La Paz, Viviana, Barrandeguy, M., Galosi, Cecilia Mónica
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Veterinarias
1B genome
Argentina
Equine herpesvirus 1
First detection
Restriction endonuclease analysis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83202
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83202
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
1B genome
Argentina
Equine herpesvirus 1
First detection
Restriction endonuclease analysis
spellingShingle Ciencias Veterinarias
1B genome
Argentina
Equine herpesvirus 1
First detection
Restriction endonuclease analysis
Martínez, J. P.
Martín Ocampos, Giselle Paula
Fernandez, L. C.
Fuentealba, Nadia Analía
Cid de La Paz, Viviana
Barrandeguy, M.
Galosi, Cecilia Mónica
Detection of equine herpesvirus 1 genome 1B in Argentina
topic_facet Ciencias Veterinarias
1B genome
Argentina
Equine herpesvirus 1
First detection
Restriction endonuclease analysis
description To determine the genomic variation of equine herpesviruses (EHVs) isolated in Argentina between 1979 and the first half of 2004, DNA sequences from all 69 strains isolated were analysed. Sixty strains were recovered from aborted fetuses, one from leucocyte-rich plasma from a horse with respiratory signs and eight from cases of neonatal disease. The DNA was extracted from rabbit kidney epithelial (RK13) cells infected with each strain and digested with three restriction endonucleases (BamHI, Bg/II and KpnI). Two strains could be differentiated using BamHI restriction and were assigned to the EHV-1 1B prototype group. Only one of these two strains was typed EHV-1 1B with Bg/II. DNA digestion with KpnI was ineffective. The results obtained in this study demonstrate that the EHV-1 1B genome has been present in Argentina since at least 1996. The finding of two strains with this electropherotype suggests that there is genomic heterogeneity among Argentinian isolates.
format Articulo
Articulo
author Martínez, J. P.
Martín Ocampos, Giselle Paula
Fernandez, L. C.
Fuentealba, Nadia Analía
Cid de La Paz, Viviana
Barrandeguy, M.
Galosi, Cecilia Mónica
author_facet Martínez, J. P.
Martín Ocampos, Giselle Paula
Fernandez, L. C.
Fuentealba, Nadia Analía
Cid de La Paz, Viviana
Barrandeguy, M.
Galosi, Cecilia Mónica
author_sort Martínez, J. P.
title Detection of equine herpesvirus 1 genome 1B in Argentina
title_short Detection of equine herpesvirus 1 genome 1B in Argentina
title_full Detection of equine herpesvirus 1 genome 1B in Argentina
title_fullStr Detection of equine herpesvirus 1 genome 1B in Argentina
title_full_unstemmed Detection of equine herpesvirus 1 genome 1B in Argentina
title_sort detection of equine herpesvirus 1 genome 1b in argentina
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/83202
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