Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida

Pfiesteria spp. are mixotrophic armored dinoflagellates populating the Atlantic coastal waters of the United States. They have been a focus of intense research due to their reported association with several fish mortality events. We have now used a clonal culture of Pfiesteria piscicida and several...

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Autores principales:	Saito, Keiko, Drgon, Tomás, Krupatkina, Danara N., Drgonova, Jana, Terlizzi, Daniel E., Mercer, Natalia, Vasta, Gerardo R.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2007
Materias:	Ciencias Exactas Pfiesteria spp encystment germination
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/83143
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-83143
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Pfiesteria spp encystment germination
spellingShingle	Ciencias Exactas Pfiesteria spp encystment germination Saito, Keiko Drgon, Tomás Krupatkina, Danara N. Drgonova, Jana Terlizzi, Daniel E. Mercer, Natalia Vasta, Gerardo R. Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida
topic_facet	Ciencias Exactas Pfiesteria spp encystment germination
description	Pfiesteria spp. are mixotrophic armored dinoflagellates populating the Atlantic coastal waters of the United States. They have been a focus of intense research due to their reported association with several fish mortality events. We have now used a clonal culture of Pfiesteria piscicida and several new environmental isolates to describe growth characteristics, feeding, and factors contributing to the encystment and germination of the organism in both laboratory and environmental samples. We also discuss applied methods of detection of the different morphological forms of Pfiesteria in environmental samples. In summary, Pfiesteria, when grown with its algal prey, Rhodomonas sp., presents a typical growth curve with lag, exponential, and stationary phases, followed by encystment. The doubling time in exponential phase is about 12 h. The profiles of proliferation under a standard light cycle and in the dark were similar, although the peak cell densities were markedly lower when cells were grown in the dark. The addition of urea, chicken manure, and soil extracts did not enhance Pfiesteria proliferation, but crude unfiltered spent aquarium water did. Under conditions of food deprivation or cold (4°C), Pfiesteria readily formed harvestable cysts that were further analyzed by PCR and scanning electron microscopy. The germination of Pfiesteria cysts in environmental sediment was enhanced by the presence of live fish: dinospores could be detected 13 to 15 days earlier and reached 5- to 10-times-higher peak cell densities with live fish than with artificial seawater or f/2 medium alone. The addition of ammonia, urea, nitrate, phosphate, or surprisingly, spent fish aquarium water had no effect.
format	Articulo Articulo
author	Saito, Keiko Drgon, Tomás Krupatkina, Danara N. Drgonova, Jana Terlizzi, Daniel E. Mercer, Natalia Vasta, Gerardo R.
author_facet	Saito, Keiko Drgon, Tomás Krupatkina, Danara N. Drgonova, Jana Terlizzi, Daniel E. Mercer, Natalia Vasta, Gerardo R.
author_sort	Saito, Keiko
title	Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida
title_short	Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida
title_full	Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida
title_fullStr	Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida
title_full_unstemmed	Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida
title_sort	effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of pfiesteria piscicida
publishDate	2007
url	http://sedici.unlp.edu.ar/handle/10915/83143
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bdutipo_str	Repositorios
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Effect of biotic and abiotic factors on in vitro proliferation, encystment, and excystment of Pfiesteria piscicida

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