Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis

<i>Enterococcus faecalis</i> is a gram-positive commensal bacterium of the human intestinal tract. Its opportunistic pathogenicity has been enhanced by the acquisition of multiple antibiotic resistances, making the treatment of enterococcal infections an increasingly difficult problem. T...

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Detalles Bibliográficos
Autores principales: Del Papa, María Florencia, Perego, Marta
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Ciencias Exactas
Enterococcus faecalis
Bacteria
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83091
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83091
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Enterococcus faecalis
Bacteria
spellingShingle Ciencias Exactas
Enterococcus faecalis
Bacteria
Del Papa, María Florencia
Perego, Marta
Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis
topic_facet Ciencias Exactas
Enterococcus faecalis
Bacteria
description <i>Enterococcus faecalis</i> is a gram-positive commensal bacterium of the human intestinal tract. Its opportunistic pathogenicity has been enhanced by the acquisition of multiple antibiotic resistances, making the treatment of enterococcal infections an increasingly difficult problem. The extraordinary capacity of this organism to colonize and survive in a wide variety of ecological niches is attributable, at least in part, to signal transduction pathways mediated by two-component systems (TCS). Here, the ability of <i>E. faecalis</i> to utilize ethanolamine as the sole carbon source is shown to be dependent upon the RR-HK17 (EF1633-EF1632) TCS. Ethanolamine is an abundant compound in the human intestine, and thus, the ability of bacteria to utilize it as a source of carbon and nitrogen may provide an advantage for survival and colonization. Growth of <i>E. faecalis</i> in a synthetic medium with ethanolamine was abolished in the response regulator RR17 mutant strain. Transcription of the response regulator gene was induced by the presence of ethanolamine. Ethanolamine induced a 15-fold increase in the rate of autophosphorylation in vitro of the HK17 sensor histidine kinase, indicating that this is the ligand recognized by the sensor domain of the kinase. These results assign a role to the RR-HK17 TCS as coordinator of the enterococcal response to specific nutritional conditions existing at the site of bacterial invasion, the intestinal tract of an animal host.
format Articulo
Articulo
author Del Papa, María Florencia
Perego, Marta
author_facet Del Papa, María Florencia
Perego, Marta
author_sort Del Papa, María Florencia
title Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis
title_short Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis
title_full Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis
title_fullStr Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis
title_full_unstemmed Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis
title_sort ethanolamine activates a sensor histidine kinase regulating its utilization in enterococcus faecalis
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/83091
work_keys_str_mv AT delpapamariaflorencia ethanolamineactivatesasensorhistidinekinaseregulatingitsutilizationinenterococcusfaecalis
AT peregomarta ethanolamineactivatesasensorhistidinekinaseregulatingitsutilizationinenterococcusfaecalis
bdutipo_str Repositorios
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